Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST

Kerry O'Donnell; Richard A. Humber; David M. Geiser; Seogchan Kang; Bongsoo Park; Vincent A.R.G. Robert; Pedro W. Crous; Peter R. Johnston; Takayuki Aoki; Alejandro P. Rooney; Stephen A. Rehner

doi:10.3852/11-179

Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST

Kerry O'Donnell, Richard A. Humber, David M. Geiser, Seogchan Kang, Bongsoo Park, Vincent A.R.G. Robert, Pedro W. Crous, Peter R. Johnston, Takayuki Aoki, Alejandro P. Rooney, Stephen A. Rehner

Research output: Contribution to journal › Article › peer-review

105 Scopus citations

Abstract

We constructed several multilocus DNA sequence datasets to assess the phylogenetic diversity of insecticolous fusaria, especially focusing on those housed at the Agricultural Research Service Collection of Entomopathogenic Fungi (ARSEF), and to aid molecular identifications of unknowns via the FUSARIUM-ID and Fusarium MLST online databases and analysis packages. Analyses of a 190-taxon, twolocus dataset, which included 159 isolates from insects, indicated that: (i) insect-associated fusaria were nested within 10 species complexes spanning the phylogenetic breadth of Fusarium, (ii) novel, putatively unnamed insecticolous species were nested within 8/10 species complexes and (iii) Latin binomials could be applied with confidence to only 18/58 phylogenetically distinct fusaria associated with pest insects. Phylogenetic analyses of an 82-taxon, three-locus dataset nearly fully resolved evolutionary relationships among the 10 clades containing insecticolous fusaria. Multilocus typing of isolates within four species complexes identified surprisingly high genetic diversity in that 63/65 of the fusaria typed represented newly discovered haplotypes. The DNA sequence data, together with corrected ABI sequence chromatograms and alignments, have been uploaded to the following websites dedicated to identifying fusaria: FUSARIUM-ID (http://isolate.fusariumdb.org) at Pennsylvania State University's Department of Plant Pathology and Fusarium MLST (http://www.cbs.knaw.nl/fusarium) at the Centraalbureau voor Schimmelcultures (CBS-KNAW) Fungal Biodiversity Center.

Original language	English (US)
Pages (from-to)	427-445
Number of pages	19
Journal	Mycologia
Volume	104
Issue number	2
DOIs	https://doi.org/10.3852/11-179
State	Published - Mar 2012
Externally published	Yes

Keywords

Ascomycota
DNA sequence
EF-1α
GCPSR
RPB1
RPB2
Species limits

ASJC Scopus subject areas

Physiology
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics
Cell Biology

Access to Document

10.3852/11-179

Cite this

O'Donnell, K., Humber, R. A., Geiser, D. M., Kang, S., Park, B., Robert, V. A. R. G., Crous, P. W., Johnston, P. R., Aoki, T., Rooney, A. P., & Rehner, S. A. (2012). Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST. Mycologia, 104(2), 427-445. https://doi.org/10.3852/11-179

@article{d8eb55f895f64116a96e51794bfe90c9,

title = "Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST",

abstract = "We constructed several multilocus DNA sequence datasets to assess the phylogenetic diversity of insecticolous fusaria, especially focusing on those housed at the Agricultural Research Service Collection of Entomopathogenic Fungi (ARSEF), and to aid molecular identifications of unknowns via the FUSARIUM-ID and Fusarium MLST online databases and analysis packages. Analyses of a 190-taxon, twolocus dataset, which included 159 isolates from insects, indicated that: (i) insect-associated fusaria were nested within 10 species complexes spanning the phylogenetic breadth of Fusarium, (ii) novel, putatively unnamed insecticolous species were nested within 8/10 species complexes and (iii) Latin binomials could be applied with confidence to only 18/58 phylogenetically distinct fusaria associated with pest insects. Phylogenetic analyses of an 82-taxon, three-locus dataset nearly fully resolved evolutionary relationships among the 10 clades containing insecticolous fusaria. Multilocus typing of isolates within four species complexes identified surprisingly high genetic diversity in that 63/65 of the fusaria typed represented newly discovered haplotypes. The DNA sequence data, together with corrected ABI sequence chromatograms and alignments, have been uploaded to the following websites dedicated to identifying fusaria: FUSARIUM-ID (http://isolate.fusariumdb.org) at Pennsylvania State University's Department of Plant Pathology and Fusarium MLST (http://www.cbs.knaw.nl/fusarium) at the Centraalbureau voor Schimmelcultures (CBS-KNAW) Fungal Biodiversity Center.",

keywords = "Ascomycota, DNA sequence, EF-1α, GCPSR, RPB1, RPB2, Species limits",

author = "Kerry O'Donnell and Humber, {Richard A.} and Geiser, {David M.} and Seogchan Kang and Bongsoo Park and Robert, {Vincent A.R.G.} and Crous, {Pedro W.} and Johnston, {Peter R.} and Takayuki Aoki and Rooney, {Alejandro P.} and Rehner, {Stephen A.}",

year = "2012",

month = mar,

doi = "10.3852/11-179",

language = "English (US)",

volume = "104",

pages = "427--445",

journal = "Mycologia",

issn = "0027-5514",

publisher = "Allen Press Inc.",

number = "2",

}

TY - JOUR

T1 - Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST

AU - O'Donnell, Kerry

AU - Humber, Richard A.

AU - Geiser, David M.

AU - Kang, Seogchan

AU - Park, Bongsoo

AU - Robert, Vincent A.R.G.

AU - Crous, Pedro W.

AU - Johnston, Peter R.

AU - Aoki, Takayuki

AU - Rooney, Alejandro P.

AU - Rehner, Stephen A.

PY - 2012/3

Y1 - 2012/3

N2 - We constructed several multilocus DNA sequence datasets to assess the phylogenetic diversity of insecticolous fusaria, especially focusing on those housed at the Agricultural Research Service Collection of Entomopathogenic Fungi (ARSEF), and to aid molecular identifications of unknowns via the FUSARIUM-ID and Fusarium MLST online databases and analysis packages. Analyses of a 190-taxon, twolocus dataset, which included 159 isolates from insects, indicated that: (i) insect-associated fusaria were nested within 10 species complexes spanning the phylogenetic breadth of Fusarium, (ii) novel, putatively unnamed insecticolous species were nested within 8/10 species complexes and (iii) Latin binomials could be applied with confidence to only 18/58 phylogenetically distinct fusaria associated with pest insects. Phylogenetic analyses of an 82-taxon, three-locus dataset nearly fully resolved evolutionary relationships among the 10 clades containing insecticolous fusaria. Multilocus typing of isolates within four species complexes identified surprisingly high genetic diversity in that 63/65 of the fusaria typed represented newly discovered haplotypes. The DNA sequence data, together with corrected ABI sequence chromatograms and alignments, have been uploaded to the following websites dedicated to identifying fusaria: FUSARIUM-ID (http://isolate.fusariumdb.org) at Pennsylvania State University's Department of Plant Pathology and Fusarium MLST (http://www.cbs.knaw.nl/fusarium) at the Centraalbureau voor Schimmelcultures (CBS-KNAW) Fungal Biodiversity Center.

AB - We constructed several multilocus DNA sequence datasets to assess the phylogenetic diversity of insecticolous fusaria, especially focusing on those housed at the Agricultural Research Service Collection of Entomopathogenic Fungi (ARSEF), and to aid molecular identifications of unknowns via the FUSARIUM-ID and Fusarium MLST online databases and analysis packages. Analyses of a 190-taxon, twolocus dataset, which included 159 isolates from insects, indicated that: (i) insect-associated fusaria were nested within 10 species complexes spanning the phylogenetic breadth of Fusarium, (ii) novel, putatively unnamed insecticolous species were nested within 8/10 species complexes and (iii) Latin binomials could be applied with confidence to only 18/58 phylogenetically distinct fusaria associated with pest insects. Phylogenetic analyses of an 82-taxon, three-locus dataset nearly fully resolved evolutionary relationships among the 10 clades containing insecticolous fusaria. Multilocus typing of isolates within four species complexes identified surprisingly high genetic diversity in that 63/65 of the fusaria typed represented newly discovered haplotypes. The DNA sequence data, together with corrected ABI sequence chromatograms and alignments, have been uploaded to the following websites dedicated to identifying fusaria: FUSARIUM-ID (http://isolate.fusariumdb.org) at Pennsylvania State University's Department of Plant Pathology and Fusarium MLST (http://www.cbs.knaw.nl/fusarium) at the Centraalbureau voor Schimmelcultures (CBS-KNAW) Fungal Biodiversity Center.

KW - Ascomycota

KW - DNA sequence

KW - EF-1α

KW - GCPSR

KW - RPB1

KW - RPB2

KW - Species limits

UR - http://www.scopus.com/inward/record.url?scp=84862313467&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862313467&partnerID=8YFLogxK

U2 - 10.3852/11-179

DO - 10.3852/11-179

M3 - Article

C2 - 22086911

AN - SCOPUS:84862313467

SN - 0027-5514

VL - 104

SP - 427

EP - 445

JO - Mycologia

JF - Mycologia

IS - 2

ER -

Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this