Photodynamic therapy for the treatment of metastatic lesions in bone: Studies in rat and porcine models

S. Burch, A. Bogaards, J. Siewerdsen, D. Moseley, A. Yee, J. Finkelstein, Robert Weersink, B. C. Wilson, S. K. Bisland

Research output: Contribution to journalArticlepeer-review

Abstract

This study represents the first reported use of photodynamic therapy (PDT) for metastatic bone lesions and specifically, as a treatment for spinal metastases. A model of bone metastasis in rat confirmed the efficacy of benzoporphyrin derivative-monoacid-mediated PDT for treating lesions within the spine and appendicular bone. Fluorimetry confirmed the selective accumulation of drug into the tumor(s) at 3 h post-injection. 48 h post-light delivery into the vertebral body of the rat spine loss of bioluminescent signal and histological analyses of sectioned spine confirmed MT-1 tumor cell kill in vivo as previously confirmed in vitro using an established cell viability assay. Porcine vertebrae provided a model comparable to that of human for light propagation and PDT response. Histological examination of vertebrae 48 h post-PDT revealed a necrotic radius of 0.6 cm with an average fluence rate of 4.3 mW/cm2. Non-necrotic tissue damage was evident up to 2 cm out from the treatment fiber. Results support the application of PDT to the treatment of primary or metastatic lesions within bone.

Original languageEnglish (US)
Article number034011
JournalJournal of biomedical optics
Volume10
Issue number3
DOIs
StatePublished - May 1 2005
Externally publishedYes

Keywords

  • Bioluminescence
  • Bone
  • Cancer
  • Dosimetry
  • Metastases
  • Photodynamic
  • Spine

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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