Photodynamic therapy for the treatment of metastatic lesions in bone: Studies in rat and porcine models

S. Burch, S. K. Bisland, J. Siewerdsen, A. Bogaards, D. Moseley, A. Yee, J. Finkelstein, B. Wilson

Research output: Contribution to journalConference articlepeer-review

Abstract

This study represents the first reported use of photodynamic therapy in bone and specifically, as a treatment for spinal metastases. A metastatic model in rat confirmed the efficacy of benzoporphyrin derivative-monoacid-mediated PDT for treating lesions within the spine and appendicular bone. Fluorimetry confirmed the selective accumulation of drug into the tumor(s) at 3 hours post-injection. 48 hrs post light delivery into the vertebral body of the rat spine loss of bioluminescent signal and histological analyses of sectioned spine confirmed MT-1 tumor cell kill in vivo as previously confirmed in vitro using an established cell viability assay. Porcine vertebrae provided a model comparable to that of human for light propagation and PDT response. Light measurements were recorded at 2.5 mm increments as the detector probe was retracted out of the vertebral body away from a diffusing fiber at 70-90° planar angle to it. At 30 minutes or Ihr post BPD-MA administration (6 mg/m 2), light (648 J, 150 mW/cm, 690 nm) was delivered to vertebrae L1 and/or L2. Vertebrae were harvested and sectioned for histology 48 hrs following PDT. Light propagation was plotted as distance (μm) from the emitting source. Results support the application of PDT to the treatment of primary or metastatic lesions within bone.

Original languageEnglish (US)
Pages (from-to)76-87
Number of pages12
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5315
DOIs
StatePublished - Oct 27 2004
Externally publishedYes
EventProgress in Biomedical Optics and Imaging - Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamics Therapy XIII - San Jose, CA, United States
Duration: Jan 24 2004Jan 25 2004

Keywords

  • Bioluminescence
  • Bone
  • Cancer
  • Dosimetry
  • Metastases
  • Photodynamic
  • Spine

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Photodynamic therapy for the treatment of metastatic lesions in bone: Studies in rat and porcine models'. Together they form a unique fingerprint.

Cite this