Photodynamic therapy for the treatment of metastatic lesions in bone: Studies in rat and porcine models

S. Burch, S. K. Bisland, Jeff Siewerdsen, A. Bogaards, D. Moseley, A. Yee, J. Finkelstein, B. Wilson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study represents the first reported use of photodynamic therapy in bone and specifically, as a treatment for spinal metastases. A metastatic model in rat confirmed the efficacy of benzoporphyrin derivative-monoacid-mediated PDT for treating lesions within the spine and appendicular bone. Fluorimetry confirmed the selective accumulation of drug into the tumor(s) at 3 hours post-injection. 48 hrs post light delivery into the vertebral body of the rat spine loss of bioluminescent signal and histological analyses of sectioned spine confirmed MT-1 tumor cell kill in vivo as previously confirmed in vitro using an established cell viability assay. Porcine vertebrae provided a model comparable to that of human for light propagation and PDT response. Light measurements were recorded at 2.5 mm increments as the detector probe was retracted out of the vertebral body away from a diffusing fiber at 70-90° planar angle to it. At 30 minutes or Ihr post BPD-MA administration (6 mg/m 2), light (648 J, 150 mW/cm, 690 nm) was delivered to vertebrae L1 and/or L2. Vertebrae were harvested and sectioned for histology 48 hrs following PDT. Light propagation was plotted as distance (μm) from the emitting source. Results support the application of PDT to the treatment of primary or metastatic lesions within bone.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsD. Kessel
Pages76-87
Number of pages12
Volume5315
DOIs
StatePublished - 2004
Externally publishedYes
EventProgress in Biomedical Optics and Imaging - Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamics Therapy XIII - San Jose, CA, United States
Duration: Jan 24 2004Jan 25 2004

Other

OtherProgress in Biomedical Optics and Imaging - Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamics Therapy XIII
CountryUnited States
CitySan Jose, CA
Period1/24/041/25/04

Fingerprint

Photodynamic therapy
lesions
bones
rats
Rats
therapy
vertebrae
Bone
Light propagation
spine
Tumors
Cells
Light measurement
Histology
tumors
Assays
histology
propagation
Derivatives
Detectors

Keywords

  • Bioluminescence
  • Bone
  • Cancer
  • Dosimetry
  • Metastases
  • Photodynamic
  • Spine

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Burch, S., Bisland, S. K., Siewerdsen, J., Bogaards, A., Moseley, D., Yee, A., ... Wilson, B. (2004). Photodynamic therapy for the treatment of metastatic lesions in bone: Studies in rat and porcine models. In D. Kessel (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 5315, pp. 76-87) https://doi.org/10.1117/12.548174

Photodynamic therapy for the treatment of metastatic lesions in bone : Studies in rat and porcine models. / Burch, S.; Bisland, S. K.; Siewerdsen, Jeff; Bogaards, A.; Moseley, D.; Yee, A.; Finkelstein, J.; Wilson, B.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / D. Kessel. Vol. 5315 2004. p. 76-87.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Burch, S, Bisland, SK, Siewerdsen, J, Bogaards, A, Moseley, D, Yee, A, Finkelstein, J & Wilson, B 2004, Photodynamic therapy for the treatment of metastatic lesions in bone: Studies in rat and porcine models. in D Kessel (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 5315, pp. 76-87, Progress in Biomedical Optics and Imaging - Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamics Therapy XIII, San Jose, CA, United States, 1/24/04. https://doi.org/10.1117/12.548174
Burch S, Bisland SK, Siewerdsen J, Bogaards A, Moseley D, Yee A et al. Photodynamic therapy for the treatment of metastatic lesions in bone: Studies in rat and porcine models. In Kessel D, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 5315. 2004. p. 76-87 https://doi.org/10.1117/12.548174
Burch, S. ; Bisland, S. K. ; Siewerdsen, Jeff ; Bogaards, A. ; Moseley, D. ; Yee, A. ; Finkelstein, J. ; Wilson, B. / Photodynamic therapy for the treatment of metastatic lesions in bone : Studies in rat and porcine models. Proceedings of SPIE - The International Society for Optical Engineering. editor / D. Kessel. Vol. 5315 2004. pp. 76-87
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