TY - JOUR
T1 - Photocoagulation does not ablate angiogenic markers in rabbit retina
AU - Campochiaro, Peter A.
AU - Blaydes, Steven H.
N1 - Funding Information:
tomimicthem through otherdevelopmeans.ways ThisinvestigationwassupportedbyPublic HealthService grant Md.EY05951from the National EyeInstitute,Bethesda,
PY - 1988/5
Y1 - 1988/5
N2 - Panretinal photocoagulation (PRP) in rabbits produces treatment areas consisting of destroyed outer retina and intact inner retina separated by areas of normalappearing retina. Intravenous iodoacetate produces widespread destruction of the outer retina with sparing of the inner retina, approximating total, confluent PRP. After PRP (at three weeks), intravenous injection of iodoacetate (at three weeks or four months), and in control rabbits, eyes were harvested and retinas were homogenized in a balanced salt solution. Retinal homogenates were measured for three markers of angiogenic activity: vascular endothelial cell migration, proliferation, and production of plasminogen activator. Retinas subjected to PRP or intravenous iodoacetate (at three weeks or four months) stimulated migration and proliferation above baseline levels twice as much as control retinas and stimulated plasminogen activator production by twofold to 2.5-fold. These data suggest that PRP does not eliminate the ability of the retina to produce angiogenic activity, but rather exerts its antiangiogenic effect in an indirect fashion.
AB - Panretinal photocoagulation (PRP) in rabbits produces treatment areas consisting of destroyed outer retina and intact inner retina separated by areas of normalappearing retina. Intravenous iodoacetate produces widespread destruction of the outer retina with sparing of the inner retina, approximating total, confluent PRP. After PRP (at three weeks), intravenous injection of iodoacetate (at three weeks or four months), and in control rabbits, eyes were harvested and retinas were homogenized in a balanced salt solution. Retinal homogenates were measured for three markers of angiogenic activity: vascular endothelial cell migration, proliferation, and production of plasminogen activator. Retinas subjected to PRP or intravenous iodoacetate (at three weeks or four months) stimulated migration and proliferation above baseline levels twice as much as control retinas and stimulated plasminogen activator production by twofold to 2.5-fold. These data suggest that PRP does not eliminate the ability of the retina to produce angiogenic activity, but rather exerts its antiangiogenic effect in an indirect fashion.
UR - http://www.scopus.com/inward/record.url?scp=0023945064&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023945064&partnerID=8YFLogxK
U2 - 10.1001/archopht.1988.01060130738035
DO - 10.1001/archopht.1988.01060130738035
M3 - Article
C2 - 2451908
AN - SCOPUS:0023945064
SN - 0003-9950
VL - 106
SP - 676
EP - 679
JO - Archives of ophthalmology
JF - Archives of ophthalmology
IS - 5
ER -