PHOTOBLEACHING DIFFERENCE ABSORPTION SPECTRA OF HUMAN CONE PIGMENTS: QUANTITATIVE ANALYSIS AND COMPARISON TO OTHER METHODS

Shannath L. Merbs, Jeremy Nathans

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract— Four human cone pigment apoproteins were expressed by transfection of human tissue culture cells with the corresponding complementary DNA clones. Following reconstitution of the cone pigments by incubation with 11‐cis retinal, photobleaching difference absorption spectra were obtained for the blue pigment, the green pigment, and two polymorphic variants of the red pigment. These spectra were analyzed to determine the wavelengths of maximal absorbance and the bandwidths. The recombinant cone pigment spectra were compared to human cone spectral sensitivities and cone pigment absorption spectra determined by microspectrophotometry, single‐cell electrophysiology, reflection densitometry, electroretinography, and psychophysical color and brightness matching.

Original languageEnglish (US)
Pages (from-to)869-881
Number of pages13
JournalPhotochemistry and Photobiology
Volume56
Issue number6
DOIs
StatePublished - Dec 1992

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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