TY - JOUR
T1 - PHOTOBLEACHING DIFFERENCE ABSORPTION SPECTRA OF HUMAN CONE PIGMENTS
T2 - QUANTITATIVE ANALYSIS AND COMPARISON TO OTHER METHODS
AU - Merbs, Shannath L.
AU - Nathans, Jeremy
PY - 1992/12
Y1 - 1992/12
N2 - Abstract— Four human cone pigment apoproteins were expressed by transfection of human tissue culture cells with the corresponding complementary DNA clones. Following reconstitution of the cone pigments by incubation with 11‐cis retinal, photobleaching difference absorption spectra were obtained for the blue pigment, the green pigment, and two polymorphic variants of the red pigment. These spectra were analyzed to determine the wavelengths of maximal absorbance and the bandwidths. The recombinant cone pigment spectra were compared to human cone spectral sensitivities and cone pigment absorption spectra determined by microspectrophotometry, single‐cell electrophysiology, reflection densitometry, electroretinography, and psychophysical color and brightness matching.
AB - Abstract— Four human cone pigment apoproteins were expressed by transfection of human tissue culture cells with the corresponding complementary DNA clones. Following reconstitution of the cone pigments by incubation with 11‐cis retinal, photobleaching difference absorption spectra were obtained for the blue pigment, the green pigment, and two polymorphic variants of the red pigment. These spectra were analyzed to determine the wavelengths of maximal absorbance and the bandwidths. The recombinant cone pigment spectra were compared to human cone spectral sensitivities and cone pigment absorption spectra determined by microspectrophotometry, single‐cell electrophysiology, reflection densitometry, electroretinography, and psychophysical color and brightness matching.
UR - http://www.scopus.com/inward/record.url?scp=0027016259&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027016259&partnerID=8YFLogxK
U2 - 10.1111/j.1751-1097.1992.tb09708.x
DO - 10.1111/j.1751-1097.1992.tb09708.x
M3 - Article
C2 - 1492133
AN - SCOPUS:0027016259
SN - 0031-8655
VL - 56
SP - 869
EP - 881
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 6
ER -