Photic regulation of arylalkylamine N-acetyltransferasel mRNA in trout retina

Kanta Mizusawa, Masayuki Iigo, Tomohiro Masuda, Katsumi Aida

Research output: Contribution to journalArticle

Abstract

Melatonin production in the pineal organ and retina is controlled by both light-dark cycles and a circadian clock via the oscillating activity of arylalkylamine N-acetyltransferase (AANAT) in most vertebrates. However, this clock regulation is absent in the rainbow trout (Oncorhynchus mykiss) pineal organ: the trout has two different AANAT genes (AANAT1 and AANAT2), and AANAT2 mRNA levels in the pineal organ did not exhibit circadian oscillation In this study, we confirmed by RT-PCR analysis that AANAT1 is expressed only in the retina, while AANAT2 is expressed in the pineal organ and brain. Real-time quantitative PCR analysis demonstrated that AANAT1 mRNA levels in the retina exhibited daily variations with high levels during the dark phase under light-dark cycles, but kept high and low titers under constant darkness and constant light, respectively. Thus, AANAT1 gene expression in the trout retina is regulated not by a circadian clock but by lighting conditions. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish (US)
Pages (from-to)3473-3477
Number of pages5
JournalNeuroreport
Volume11
Issue number16
DOIs
StatePublished - Nov 9 2000

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Keywords

  • Arylalkylamine N-acetyltransferase
  • Circadian clock
  • Light-dark cycles
  • Melatonin
  • Rainbow trout
  • Real-time quantitative PCR
  • Retina

ASJC Scopus subject areas

  • Neuroscience(all)

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