Phosphorylation-related immunoreactivity and the rate of transport of neurofilaments in chronic 2,5-hexanedione intoxication

Danny F. Watson, Kenneth P. Fittro, Paul N. Hoffman, John W. Griffin

Research output: Contribution to journalArticlepeer-review

Abstract

Axonal transport of neurofilaments and the phosphorylation of epitopes on neurofilament proteins was studied in rats chronically intoxicated with 2,5-hexanedione. Sensory axons arising from the L5 dorsal root ganglion exhibited accelerated transport and a reduced abundance of neurofilament proteins. The binding of an antibody to phosphorylated neurofilament epitopes was compared to the binding of an antibody to non-phosphorylated epitopes by quantitative ELISA. This immunochemical index of neurofilament phosphorylation was reduced in dorsal roots, proximal peripheral sensory axons and ventral roots, but not in a distal nerve (the nerve to the soleus). Axotomy produced a reduction in neurofilament protein abundance comparable to hexanedione without any change in the immunochemical phosphorulation index. These results are consistent with the hypothesis that the state of phosphorylation of neurofilaments in proximal axons is correlated with the rate of neurofilament transport.

Original languageEnglish (US)
Pages (from-to)103-109
Number of pages7
JournalBrain research
Volume539
Issue number1
DOIs
StatePublished - Jan 18 1991

Keywords

  • 2,5-Hexanedione
  • Axonal transport
  • Axotomy
  • Immunoassay
  • Neurofilament
  • Phosphorylation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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