Phosphorylation of proteins by inositol pyrophosphates

Adolfo Saiardi; Rashna Bhandari; Adam C. Resnick; Adele M. Snowman; Solomon H. Snyder

doi:10.1126/science.1103344

Phosphorylation of proteins by inositol pyrophosphates

Adolfo Saiardi, Rashna Bhandari, Adam C. Resnick, Adele M. Snowman, Solomon H. Snyder

School of Medicine

Research output: Contribution to journal › Article › peer-review

225 Scopus citations

Abstract

The inositol pyrophosphates IP₇ and IP₈ contain highly energetic pyrophosphate bonds. Although implicated in various biologic functions, their molecular sites of action have not been clarified. Using radiolabeled IP₇, we detected phosphorylation of multiple eukaryotic proteins. We also observed phosphorylation of endogenous proteins by endogenous IP₇ in yeast. Phosphorylation by IP₇ is nonenzymatic and may represent a novel intracellular signaling mechanism.

Original language	English (US)
Pages (from-to)	2101-2105
Number of pages	5
Journal	Science
Volume	306
Issue number	5704
DOIs	https://doi.org/10.1126/science.1103344
State	Published - Dec 17 2004

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title = "Phosphorylation of proteins by inositol pyrophosphates",

abstract = "The inositol pyrophosphates IP7 and IP8 contain highly energetic pyrophosphate bonds. Although implicated in various biologic functions, their molecular sites of action have not been clarified. Using radiolabeled IP7, we detected phosphorylation of multiple eukaryotic proteins. We also observed phosphorylation of endogenous proteins by endogenous IP7 in yeast. Phosphorylation by IP7 is nonenzymatic and may represent a novel intracellular signaling mechanism.",

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AU - Saiardi, Adolfo

AU - Bhandari, Rashna

AU - Resnick, Adam C.

AU - Snowman, Adele M.

AU - Snyder, Solomon H.

PY - 2004/12/17

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AB - The inositol pyrophosphates IP7 and IP8 contain highly energetic pyrophosphate bonds. Although implicated in various biologic functions, their molecular sites of action have not been clarified. Using radiolabeled IP7, we detected phosphorylation of multiple eukaryotic proteins. We also observed phosphorylation of endogenous proteins by endogenous IP7 in yeast. Phosphorylation by IP7 is nonenzymatic and may represent a novel intracellular signaling mechanism.

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Phosphorylation of proteins by inositol pyrophosphates

Abstract

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