TY - JOUR
T1 - Phosphorylation of chemoattractant receptors is not essential for chemotaxis or termination of G-protein-mediated responses
AU - Kim, Ji Yun
AU - Soede, Ron D.M.
AU - Schaap, Pauline
AU - Valkema, Romi
AU - Borleis, Jane A.
AU - Van Haastert, Peter J.M.
AU - Devreotes, Peter N.
AU - Hereld, Dale
PY - 1997
Y1 - 1997
N2 - In several G-protein-coupled signaling systems, ligand-induced receptor phosphorylation by specific kinases is suggested to lead to desensitization via mechanisms including receptor/G-protein uncoupling, receptor internalization, and receptor down-regulation. We report here that elimination of phosphorylation of a chemoattractant receptor of Dictyostelium, either by site-directed substitution of the serines or by truncation of the C-terminal cytoplasmic domain, completely prevented agonist-induced loss of ligand binding but did not impair the adaptation of several receptor-mediated responses including the activation of adenylyl and guanylyl cyclases and actin polymerization. In addition, the phosphorylation- deficient receptors were capable of mediating chemotaxis, aggregation, and differentiation. We propose that for chemoattractant receptors agonist- induced phosphorylation regulates surface binding activity but other phosphorylation-independent mechanisms mediate response adaptation.
AB - In several G-protein-coupled signaling systems, ligand-induced receptor phosphorylation by specific kinases is suggested to lead to desensitization via mechanisms including receptor/G-protein uncoupling, receptor internalization, and receptor down-regulation. We report here that elimination of phosphorylation of a chemoattractant receptor of Dictyostelium, either by site-directed substitution of the serines or by truncation of the C-terminal cytoplasmic domain, completely prevented agonist-induced loss of ligand binding but did not impair the adaptation of several receptor-mediated responses including the activation of adenylyl and guanylyl cyclases and actin polymerization. In addition, the phosphorylation- deficient receptors were capable of mediating chemotaxis, aggregation, and differentiation. We propose that for chemoattractant receptors agonist- induced phosphorylation regulates surface binding activity but other phosphorylation-independent mechanisms mediate response adaptation.
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U2 - 10.1074/jbc.272.43.27313
DO - 10.1074/jbc.272.43.27313
M3 - Article
C2 - 9341180
AN - SCOPUS:0030766477
SN - 0021-9258
VL - 272
SP - 27313
EP - 27318
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 43
ER -