Phosphorylation changes induced by cAMP derivatives in the CHO cell and selected mutants

Edward G. Gabrielson; Charles Scoggin; Theodore T. Puck

doi:10.1016/0014-4827(82)90409-8

Phosphorylation changes induced by cAMP derivatives in the CHO cell and selected mutants

Edward G. Gabrielson, Charles Scoggin, Theodore T. Puck

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Abstract

Phosphorylation analysis of CHO mutants selected for unresponsiveness to the Reverse Transformation reaction of cAMP derivatives has been carried out by 2D gel electrophoresis in hopes of finding mutants differing from the wild-type cell in a minimal number of such phosphorylations. Seven differences in protein phosphorylations in the parental CHO cell have been identified as a result of treatment with db-cAMP. db-cAMP-unresponsive mutants of two kinds have been found: One type has lost all seven of the phosphorylation changes induced by db-cAMP in the wild-type cell. The other type which is equally resistant to the Reverse Transformation response differs from the parental cell in only one (or possibly two) phosphorylation(s) involving a 55 000 D protein. This protein may, therefore, be directly related to the events of Reverse Transformation.

Original language	English (US)
Pages (from-to)	63-68
Number of pages	6
Journal	Experimental cell research
Volume	142
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(82)90409-8
State	Published - Nov 1982
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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@article{79607c45beb94695a5a7a68b21e8f260,

title = "Phosphorylation changes induced by cAMP derivatives in the CHO cell and selected mutants",

abstract = "Phosphorylation analysis of CHO mutants selected for unresponsiveness to the Reverse Transformation reaction of cAMP derivatives has been carried out by 2D gel electrophoresis in hopes of finding mutants differing from the wild-type cell in a minimal number of such phosphorylations. Seven differences in protein phosphorylations in the parental CHO cell have been identified as a result of treatment with db-cAMP. db-cAMP-unresponsive mutants of two kinds have been found: One type has lost all seven of the phosphorylation changes induced by db-cAMP in the wild-type cell. The other type which is equally resistant to the Reverse Transformation response differs from the parental cell in only one (or possibly two) phosphorylation(s) involving a 55 000 D protein. This protein may, therefore, be directly related to the events of Reverse Transformation.",

author = "Gabrielson, {Edward G.} and Charles Scoggin and Puck, {Theodore T.}",

note = "Funding Information: We thank Drs Ray Erikson and Masaru Imada for technical advice, and Carol Potera for editorial assistance. This is contribution number 387 of the Eleanor Roosevelt Institute for Cancer Research. This work was supported by grants from The Milheim Foundation no. 344898, ACS-PRP-32, and NIH-HD02080. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1982",

month = nov,

doi = "10.1016/0014-4827(82)90409-8",

language = "English (US)",

volume = "142",

pages = "63--68",

journal = "Experimental cell research",

issn = "0014-4827",

publisher = "Academic Press Inc.",

number = "1",

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T1 - Phosphorylation changes induced by cAMP derivatives in the CHO cell and selected mutants

AU - Gabrielson, Edward G.

AU - Scoggin, Charles

AU - Puck, Theodore T.

N1 - Funding Information: We thank Drs Ray Erikson and Masaru Imada for technical advice, and Carol Potera for editorial assistance. This is contribution number 387 of the Eleanor Roosevelt Institute for Cancer Research. This work was supported by grants from The Milheim Foundation no. 344898, ACS-PRP-32, and NIH-HD02080. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1982/11

Y1 - 1982/11

N2 - Phosphorylation analysis of CHO mutants selected for unresponsiveness to the Reverse Transformation reaction of cAMP derivatives has been carried out by 2D gel electrophoresis in hopes of finding mutants differing from the wild-type cell in a minimal number of such phosphorylations. Seven differences in protein phosphorylations in the parental CHO cell have been identified as a result of treatment with db-cAMP. db-cAMP-unresponsive mutants of two kinds have been found: One type has lost all seven of the phosphorylation changes induced by db-cAMP in the wild-type cell. The other type which is equally resistant to the Reverse Transformation response differs from the parental cell in only one (or possibly two) phosphorylation(s) involving a 55 000 D protein. This protein may, therefore, be directly related to the events of Reverse Transformation.

AB - Phosphorylation analysis of CHO mutants selected for unresponsiveness to the Reverse Transformation reaction of cAMP derivatives has been carried out by 2D gel electrophoresis in hopes of finding mutants differing from the wild-type cell in a minimal number of such phosphorylations. Seven differences in protein phosphorylations in the parental CHO cell have been identified as a result of treatment with db-cAMP. db-cAMP-unresponsive mutants of two kinds have been found: One type has lost all seven of the phosphorylation changes induced by db-cAMP in the wild-type cell. The other type which is equally resistant to the Reverse Transformation response differs from the parental cell in only one (or possibly two) phosphorylation(s) involving a 55 000 D protein. This protein may, therefore, be directly related to the events of Reverse Transformation.

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JF - Experimental cell research

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Phosphorylation changes induced by cAMP derivatives in the CHO cell and selected mutants

Abstract

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