Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease

Penelope G. Foulds, J. Douglas Mitchell, Angela Parker, Roisin Turner, Gerwyn Green, Peter Diggle, Masato Hasegawa, Mark Taylor, David Mann, David Allsop

Research output: Contribution to journalArticle

Abstract

Parkinson's disease (PD) is characterized by the presence of Lewy bodies containing phosphorylated and aggregated α-synuclein (α-syn). α-Syn is present in human body fluids, including blood plasma, and is a potential biomarker for PD. Immunoassays for total and oligomeric forms of both normal and phosphorylated (at Ser-129) α-syn have been used to assay plasma samples from a longitudinal cohort of 32 patients with PD (sampled at mo 0, 1, 2, 3), as well as single plasma samples from a group of 30 healthy control participants. The levels of α-syn in plasma varied greatly between individuals, but were remarkably consistent over time within the same individual with PD. The mean level of phospho-α-syn was found to be higher (P=0.053) in the PD samples than the controls, whereas this was not the case for total α-syn (P=0.244), oligo-α-syn (P=0.221), or oligo-phospho-α-syn (P=0.181). Immunoblots of plasma revealed bands (at 21, 24, and 50-60 kDa) corresponding to phosphorylated α-syn. Thus, phosphorylated α-syn can be detected in blood plasma and shows more promise as a diagnostic marker than the nonphosphorylated protein. Longitudinal studies undertaken over a more extended time period will be required to determine whether α-syn can act as a marker of disease progression.

Original languageEnglish (US)
Pages (from-to)4127-4137
Number of pages11
JournalFASEB Journal
Volume25
Issue number12
DOIs
StatePublished - Dec 2011
Externally publishedYes

Fingerprint

Synucleins
Biomarkers
Parkinson Disease
Blood
Plasmas
Lewy Bodies
Body fluids
Body Fluids
Human Body
Immunoassay
Longitudinal Studies
Disease Progression
Assays
Healthy Volunteers

Keywords

  • Immunoassay
  • Immunoblot
  • Lewy body
  • Oligomer

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Foulds, P. G., Mitchell, J. D., Parker, A., Turner, R., Green, G., Diggle, P., ... Allsop, D. (2011). Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease. FASEB Journal, 25(12), 4127-4137. https://doi.org/10.1096/fj.10-179192

Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease. / Foulds, Penelope G.; Mitchell, J. Douglas; Parker, Angela; Turner, Roisin; Green, Gerwyn; Diggle, Peter; Hasegawa, Masato; Taylor, Mark; Mann, David; Allsop, David.

In: FASEB Journal, Vol. 25, No. 12, 12.2011, p. 4127-4137.

Research output: Contribution to journalArticle

Foulds, PG, Mitchell, JD, Parker, A, Turner, R, Green, G, Diggle, P, Hasegawa, M, Taylor, M, Mann, D & Allsop, D 2011, 'Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease', FASEB Journal, vol. 25, no. 12, pp. 4127-4137. https://doi.org/10.1096/fj.10-179192
Foulds, Penelope G. ; Mitchell, J. Douglas ; Parker, Angela ; Turner, Roisin ; Green, Gerwyn ; Diggle, Peter ; Hasegawa, Masato ; Taylor, Mark ; Mann, David ; Allsop, David. / Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease. In: FASEB Journal. 2011 ; Vol. 25, No. 12. pp. 4127-4137.
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