Phospholipase D regulates calcium oscillation frequency and nuclear factor-κB activity in histamine-stimulated human endothelial cells

Qinghua Hu, Roy C. Ziegelstein, Viswanathan Natarajan

Research output: Contribution to journalArticlepeer-review

Abstract

Histamine stimulates [Ca2+]i oscillations in human aortic endothelial cells (HAEC), the frequency of which regulates the activity of nuclear factor-κB (NF-κB). This study was performed to determine whether phospholipase D (PLD) is involved in this signaling pathway. At a concentration of 1 μM, which stimulates [Ca2+]i oscillations in this cell type, histamine initiated a twofold increase in [32P]phosphatidybutanol (PBt), an index of PLD activity as early as 5 min after stimulation. During established [Ca2+]i oscillations induced by 1 μM histamine, 0.3% n-butanol, which "functionally" redirects phosphatidic acid formed by PLD to PBt, decreased [Ca2+]i oscillation frequency by ∼50% and produced a similar reduction in NF-κB activity. In the presence of the inositol 1, 4, 5-trisphosphate receptor blocker xesto-spongin C, which itself decreases the frequency of histamine-stimulated [Ca2+]i oscillations, n-butanol produced a further decrease in oscillation frequency that was not associated with an additional reduction in NF-κB activity. This study shows that activation of PLD by histamine regulates [Ca2+]i oscillation frequency and NF-κB activity in HAEC.

Original languageEnglish (US)
Pages (from-to)325-332
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume292
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Calcium
  • Endothelium
  • Inflammation
  • Inositol 1, 4, 5-trisphosphate
  • Nuclear factor-κB
  • Phospholipase D
  • Xestospongin C

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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