Phospholipase C-mediated calcium signalling is required for fungal development and pathogenicity in Magnaporthe oryzae

Hee Sool Rho, Junhyun Jeon, Yong Hwan Lee

Research output: Contribution to journalArticle

Abstract

Calcium signalling has profound implications in the fungal infection of plants and animals, during which a series of physiological and morphological transitions are required. In this article, using a model fungal pathogen, Magnaporthe oryzae, we demonstrate that the regulation of the intracellular calcium concentration (Ca2+int) is essential for fungal development and pathogenesis. Imaging of Ca2+int showed that infection-specific morphogenesis is highly correlated with the spatiotemporal regulation of calcium flux. Deletion of the fungal phospholipase C gene (M. oryzae phospholipase C 1, MoPLC1) suppressed calcium flux, resulting in a fungus defective in developmental steps, including appressorium formation and pathogenicity. Surprisingly, the PLC-δ1 gene of mouse was able to functionally substitute for MoPLC1 by restoring the calcium flux, suggesting the evolutionary conservation of the phospholipase C-mediated regulation of calcium flux. Our results reveal that MoPLC1 is a conserved modulator of calcium flux that is essential for the regulation of key steps in fungal development and pathogenesis.

Original languageEnglish (US)
Pages (from-to)337-346
Number of pages10
JournalMolecular Plant Pathology
Volume10
Issue number3
DOIs
StatePublished - May 2009
Externally publishedYes

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Magnaporthe
Magnaporthe oryzae
Calcium Signaling
phospholipase C
pathogenicity
Type C Phospholipases
Virulence
calcium
Calcium
pathogenesis
Mycoses
Morphogenesis
gene
morphogenesis
Genes
appressoria
Oryza
calcium signaling
Fungi
infection

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Soil Science
  • Molecular Biology

Cite this

Phospholipase C-mediated calcium signalling is required for fungal development and pathogenicity in Magnaporthe oryzae. / Rho, Hee Sool; Jeon, Junhyun; Lee, Yong Hwan.

In: Molecular Plant Pathology, Vol. 10, No. 3, 05.2009, p. 337-346.

Research output: Contribution to journalArticle

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