@article{0d24f0a8cb6341f48ad58f213b252a8b,
title = "Phospholipase Cγ1 controls surface expression of TRPC3 through an intermolecular PH domain",
abstract = "Many ion channels are regulated by lipids, but prominent motifs for lipid binding have not been identified in most ion channels. Recently, we reported that phospholipase Cγ1 (PLC-γ1) binds to and regulates TRPC3 channels, components of agonist-induced Ca2+ entry into cells. This interaction requires a domain in PLC-γ1 that includes a partial pleckstrin homology (PH) domain-a consensus lipid-binding and protein-binding sequence. We have developed a gestalt algorithm to detect hitherto 'invisible' PH and PH-like domains, and now report that the partial PH domain of PLC-γ1 interacts with a complementary partial PH-like domain in TRPC3 to elicit lipid binding and cell-surface expression of TRPC3. Our findings imply a far greater abundance of PH domains than previously appreciated, and suggest that intermolecular PH-like domains represent a widespread signalling mode.",
author = "{Van Rossum}, {Damian B.} and Patterson, {Randen L.} and Sumit Sharma and Barrow, {Roxanne K.} and Michael Kornberg and Gill, {Donald L.} and Snyder, {Solomon H.}",
note = "Funding Information: Acknowledgements We thank D. Boehning, G. Caraveo, J. Kendall, A. Resnick and R. E. Rothe for discussion; P.-G. Suh for the gift of the PLC-g1 antibody; and B. VanRossum for graphics. This research was supported by US Public Health Service Grants and a Research Scientist Award (to S.H.S.), a National Institutes of Health Grant (to D.L.G.), and National Research Service Awards (to R.L.P.). Funding Information: for worm freezing. Some strains were supplied by the Caenorhabditis Genetic Center (CGC), which is supported by the National Institute of Health National Center for Research Resources. This work was supported by grants from the Swiss National Science Foundation, The Ernst Hadorn Foundation and the European Union (FP5 project APOCLEAR) to M.O.H., and by the Max Planck Society and an EU TMR Research Network grant to R.S.",
year = "2005",
month = mar,
day = "3",
doi = "10.1038/nature03340",
language = "English (US)",
volume = "434",
pages = "99--104",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7029",
}