Phosphoinositide signaling - Triggering actin comets versus membrane ruffles: Distinctive effects of phosphoinositides on actin reorganization

Tasuku Ueno, Björn H. Falkenburger, Christopher Pohlmeyer, Takanari Inoue

Research output: Contribution to journalArticlepeer-review

Abstract

A limited set of phosphoinositide membrane lipids regulate diverse cellular functions including proliferation, differentiation, and migration. We developed two techniques based on rapamycin-induced protein dimerization to rapidly change the concentration of plasma membrane phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]. First, using a membrane-recruitable form of PI(4)P 5-kinase, we increased PI(4,5)P2 synthesis from phosphatidylinositol 4-phosphate [PI(4)P] and found that COS-7, HeLa, and human embryonic kidney 293 cells formed bundles of motile actin filaments known as actin comets. In contrast, a second technique that increased the concentration of PI(4,5)P2 without consuming PI(4)P induced membrane ruffles. These distinct phenotypes were mediated by dynamin-mediated vesicular trafficking and mutually inhibitory crosstalk between the small guanosine triphosphatases Rac and RhoA. Our results indicate that the effect of PI(4,5)P2 on actin reorganization depends on the abundance of other phosphoinositides, such as PI(4)P. Thus, combinatorial regulation of phosphoinositide concentrations may contribute to the diversity of phosphoinositide functions.

Original languageEnglish (US)
Article numberra87
JournalScience signaling
Volume4
Issue number203
DOIs
StatePublished - Dec 13 2011

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Phosphoinositide signaling - Triggering actin comets versus membrane ruffles: Distinctive effects of phosphoinositides on actin reorganization'. Together they form a unique fingerprint.

Cite this