TY - JOUR
T1 - Phpsphoinositide second messenger system is enriched in striosomes
T2 - Immunohistochemical demonstration of inositol 1,4,5-trisphosphate receptors and phospholipase C β and γ in primate basal ganglia
AU - Fotuhi, Majid
AU - Dawson, Ted M.
AU - Sharp, Alan H.
AU - Martin, Lee J.
AU - Graybiel, Ann M.
AU - Snyder, Solomon H.
PY - 1993
Y1 - 1993
N2 - The neurochemical organization of the basal ganglia has been studied extensively with respect to neurotransmitters, neuropeptides, and their receptors. The chemoarchitecture of the striatum has been found particularly striking, because it distinguishes many substances by their relative distributions within the striosome and matrix compartments of the striatum. Very little is yet known about the differential distribution of second messenger systems in the basal ganglia, however, and no information is available about whether the distribution of second messenger systems is related to the prominent neurochemical compartmentalization of the striatum. We have examined the distribution of the phosphoinositide second messenger system in the primate basal ganglia and substantia nigra, as detected with polyclonal antisera against the inositol 1,4,5-trisphosphate receptor (IP3R), and monoclonal antisera against phospholipase C β (PLCβ) and phospholipase C γ (PLCγ). In the striatum, immunostaining for each of the three proteins was present predominantly in medium-sized neuronal perikarya and in the neuropil. Circumscribed zones of enhanced IP3R, PLCβ, and PLCγ immunoreactivity appeared in a background of generally weaker staining, and these zones corresponded to striosomes as identified by calbindin D28K and substance P immunostaining in adjacent sections. Thus, the richest representation of the phosphoinositide system in the primate striatum appears to be in striosomes. In the substantia nigra pars compacta, neurons and neuropil were immunopositive, but in the substantia nigra pars reticulata and in each segment of the globus pallidus, immunostaining was mainly confined to the neuropil. Perikaryal PCLγ immunoreactivity in the absence of detectable PLCβ or IP3R immunolabeling was found in the magnocellular neu-rons embedded in the medullary layer between the putamen and the globus pallidus. These observations demonstrate that the phosphoinositide second messenger system is selectively enhanced in neuronal subsystems of the basal ganglia, including striosomes, and suggest that signaling by phosphoinositide pathways elicits discrete effects on input-output processing by the basal ganglia.
AB - The neurochemical organization of the basal ganglia has been studied extensively with respect to neurotransmitters, neuropeptides, and their receptors. The chemoarchitecture of the striatum has been found particularly striking, because it distinguishes many substances by their relative distributions within the striosome and matrix compartments of the striatum. Very little is yet known about the differential distribution of second messenger systems in the basal ganglia, however, and no information is available about whether the distribution of second messenger systems is related to the prominent neurochemical compartmentalization of the striatum. We have examined the distribution of the phosphoinositide second messenger system in the primate basal ganglia and substantia nigra, as detected with polyclonal antisera against the inositol 1,4,5-trisphosphate receptor (IP3R), and monoclonal antisera against phospholipase C β (PLCβ) and phospholipase C γ (PLCγ). In the striatum, immunostaining for each of the three proteins was present predominantly in medium-sized neuronal perikarya and in the neuropil. Circumscribed zones of enhanced IP3R, PLCβ, and PLCγ immunoreactivity appeared in a background of generally weaker staining, and these zones corresponded to striosomes as identified by calbindin D28K and substance P immunostaining in adjacent sections. Thus, the richest representation of the phosphoinositide system in the primate striatum appears to be in striosomes. In the substantia nigra pars compacta, neurons and neuropil were immunopositive, but in the substantia nigra pars reticulata and in each segment of the globus pallidus, immunostaining was mainly confined to the neuropil. Perikaryal PCLγ immunoreactivity in the absence of detectable PLCβ or IP3R immunolabeling was found in the magnocellular neu-rons embedded in the medullary layer between the putamen and the globus pallidus. These observations demonstrate that the phosphoinositide second messenger system is selectively enhanced in neuronal subsystems of the basal ganglia, including striosomes, and suggest that signaling by phosphoinositide pathways elicits discrete effects on input-output processing by the basal ganglia.
KW - Phosphoinositide
KW - Phospholipase C
KW - Primate
KW - Striatum
KW - Striosome
UR - http://www.scopus.com/inward/record.url?scp=0027291057&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027291057&partnerID=8YFLogxK
M3 - Article
C2 - 8393481
AN - SCOPUS:0027291057
SN - 0270-6474
VL - 13
SP - 3300
EP - 3308
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 8
ER -