Phosphatidylinositol 3-Kinase/Akt pathway targets acetylation of Smad3 through Smad3/CREB-binding protein interaction. Contribution to transforming growth factor β1-induced Epstein-Barr virus reactivation

Lassad Oussaief, Aurélie Hippocrate, Vanessa Ramirez, Aurore Rampanou, Wei Zhang, David J Meyers, Philip Cole, Ridha Khelifa, Irène Joab

Research output: Contribution to journalArticle

Abstract

Epstein-Barr virus, a ubiquitous human herpesvirus, is associated with the development of carcinomas and lymphomas. We previously showed that transforming growth factor β1 (TGF-β1) mediated the virus to enter the lytic cycle, which is triggered by expression of Z Epstein-Barr virus replication activator (ZEBRA), through the ERK 1/2 MAPK signaling pathway. We report here that Akt, activated downstream from ERK 1/2, was required for TGF-β1-induced ZEBRA expression and enabled Smad3, a mediator of TGF-β1 signaling, to be acetylated by direct interaction with the co-activator CREB-binding protein and then to regulate TGF-β1-induced ZEBRA expression.

Original languageEnglish (US)
Pages (from-to)23912-23924
Number of pages13
JournalJournal of Biological Chemistry
Volume284
Issue number36
DOIs
StatePublished - Sep 4 2009

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CREB-Binding Protein
Phosphatidylinositol 3-Kinase
Acetylation
Transforming Growth Factors
Human Herpesvirus 4
Viruses
Virus Replication
Herpesviridae
Lymphoma
Carcinoma

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Phosphatidylinositol 3-Kinase/Akt pathway targets acetylation of Smad3 through Smad3/CREB-binding protein interaction. Contribution to transforming growth factor β1-induced Epstein-Barr virus reactivation. / Oussaief, Lassad; Hippocrate, Aurélie; Ramirez, Vanessa; Rampanou, Aurore; Zhang, Wei; Meyers, David J; Cole, Philip; Khelifa, Ridha; Joab, Irène.

In: Journal of Biological Chemistry, Vol. 284, No. 36, 04.09.2009, p. 23912-23924.

Research output: Contribution to journalArticle

Oussaief, Lassad ; Hippocrate, Aurélie ; Ramirez, Vanessa ; Rampanou, Aurore ; Zhang, Wei ; Meyers, David J ; Cole, Philip ; Khelifa, Ridha ; Joab, Irène. / Phosphatidylinositol 3-Kinase/Akt pathway targets acetylation of Smad3 through Smad3/CREB-binding protein interaction. Contribution to transforming growth factor β1-induced Epstein-Barr virus reactivation. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 36. pp. 23912-23924.
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