Phenotype of Cloned Mice: Development, Behavior, and Physiology

Kellie L.K. Tamashiro, Teruhiko Wakayama, Yukiko Yamazaki, Hidenori Akutsu, Stephen C. Woods, Sylvia Kondo, Ryuzo Yanagimachi, Randall R. Sakai

Research output: Contribution to journalArticle

Abstract

Cloning technology has potential to be a valuable tool in basic research, clinical medicine, and agriculture. However, it is critical to determine the consequences of this technique in resulting offspring before widespread use of the technology. Mammalian cloning using somatic cells was first demonstrated in sheep in 1997 and since then has been extended to a number of other species. We examined development, behavior, physiology, and longevity in B6C3F1 female mice cloned from adult cumulus cells. Control mice were naturally fertilized embryos subjected to the same in vitro manipulation and culture conditions as clone embryos. Clones attained developmental milestones similar to controls. Activity level, motor ability and coordination, and learning and memory skills of cloned mice were comparable with controls. Interestingly, clones gained more body weight than controls during adulthood. Increased body weight was attributable to higher body fat and was associated with hyperleptinemia and hyperinsulinemia indicating that cloned mice are obese. Cloned mice were not hyperphagic as adults and had hypersensitive leptin and melanocortin signaling systems. Longevity of cloned mice was comparable with that reported by the National Institute on Aging and the causes of death were typical for this strain of mouse. These studies represent the first comprehensive set of data to characterize cloned mice and provide critical information about the long term effects of somatic cell cloning.

Original languageEnglish (US)
Pages (from-to)1193-1200
Number of pages8
JournalExperimental Biology and Medicine
Volume228
Issue number10
DOIs
StatePublished - Nov 2003

Keywords

  • Development
  • Longevity
  • Mouse
  • Obesity
  • Somatic cell cloning

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Tamashiro, K. L. K., Wakayama, T., Yamazaki, Y., Akutsu, H., Woods, S. C., Kondo, S., Yanagimachi, R., & Sakai, R. R. (2003). Phenotype of Cloned Mice: Development, Behavior, and Physiology. Experimental Biology and Medicine, 228(10), 1193-1200. https://doi.org/10.1177/153537020322801015