Phencyclidine (PCP) receptors: autoradiographic localization in brain with the selective ligand, [3H]TCP

Andrew L. Gundlach, Brian L. Largent, Solomon H. Snyder

Research output: Contribution to journalArticle

Abstract

Receptor binding sites for the phencyclidine (PCP) analogue, [3H]TCP, have been localized in the rat and guinea pig central nervous systems by in vitro autoradiography. Quantitation of [3H]TCP binding site densities in rat brain reveals highest levels in the forebrain, in particular the strata oriens and radiatum of the hippocampus, the molecular layer of the dentate gyrus and superficial layers of the cerebral cortex. Moderate levels of binding occur in the amygdala, thalamus, anterior olfactory nucleus external plexiform layer of the olfactory bulb, olfactory tubercle, geniculate nuclei and deep layers of the cortex. Low levels of binding occur throughout most of the septum, diagonal band, hypothalamus, pons-medulla and cerebellum. Spinal cord grey matter also has low levels binding. Excitotoxin lesions of the hippocampal formation, which destroy the pyramidal and granule cells, reduce the binding of [3H]TCP to strata radiatum and oriens and the molecular layer of the dentage gyrus by 60% suggesting that [3H]TCP labels intrinsic neurons in these regions. Residual binding is probably on afferent terminals. Ibotenic acid lesions of the caudate-putamen reduce [3H]TCP binding by 70%, indicating that binding sites are localized on intrinsic striatal neurons. 6-Hydroxydopamine lesions do not alter [3H]TCP binding levels the caudate, suggesting the absence of binding sites on dopaminergic terminals in the caudate.

Original languageEnglish (US)
Pages (from-to)266-279
Number of pages14
JournalBrain research
Volume386
Issue number1-2
DOIs
StatePublished - Oct 29 1986

Keywords

  • Central nervous system
  • Lesion
  • Phencyclidine (PCP)
  • Psychotomimetic
  • Quantitative autoradiography
  • Receptor binding site
  • [H]TCP

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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