Phase separation of the receptor for immunoglobulin E and its subunits in Triton X-114

G. Alcaraz, J. P. Kinet, N. Kumar, S. A. Wank, H. Metzger

Research output: Contribution to journalArticle

Abstract

Above its critical micelle concentration, Triton X-114 in solution forms two phases at room temperature: a lower phase containing supramicellar aggregates and an upper phase largely depleted of detergent. This property of the detergent is potentially useful for separating under mild conditions proteins that bind detergent from those that do not (Bordier, C. (1981) J. Biol. Chem. 256, 1604-1607). We studied the distribution of the receptor for immunoglobulin E (IgE) and its subunits in the two phases. IgE and IgE complexed either with intact receptors or with the γa chains of the receptor alone are principally partitioned into the upper phase, whereas the unliganded receptor as well as the isolated α, and especially the β and γ chains of the receptor, preferentially partition into the lower detergent phase. Chromatography of IgE and of the subunits of the receptor on a hydrophobic support showed that the β and γ chains have a considerably greater hydrophobic surface than the α chains or IgE. These results indicate that the distribution of a protein in the two phases of phase-separated Triton X-114 is not an all-or-none effect based upon whether it binds detergent or not. Rather, it reflects the overall balance between the hydrophobic and hydrophilic properties of the protein's surface.

Original languageEnglish (US)
Pages (from-to)14922-14927
Number of pages6
JournalJournal of Biological Chemistry
Volume259
Issue number23
StatePublished - 1984
Externally publishedYes

Fingerprint

IgE Receptors
Phase separation
Detergents
Immunoglobulin E
Immunoglobulin Subunits
Surface Properties
Critical micelle concentration
Micelles
Chromatography
Membrane Proteins
Proteins
Nonidet P-40
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Alcaraz, G., Kinet, J. P., Kumar, N., Wank, S. A., & Metzger, H. (1984). Phase separation of the receptor for immunoglobulin E and its subunits in Triton X-114. Journal of Biological Chemistry, 259(23), 14922-14927.

Phase separation of the receptor for immunoglobulin E and its subunits in Triton X-114. / Alcaraz, G.; Kinet, J. P.; Kumar, N.; Wank, S. A.; Metzger, H.

In: Journal of Biological Chemistry, Vol. 259, No. 23, 1984, p. 14922-14927.

Research output: Contribution to journalArticle

Alcaraz, G, Kinet, JP, Kumar, N, Wank, SA & Metzger, H 1984, 'Phase separation of the receptor for immunoglobulin E and its subunits in Triton X-114', Journal of Biological Chemistry, vol. 259, no. 23, pp. 14922-14927.
Alcaraz G, Kinet JP, Kumar N, Wank SA, Metzger H. Phase separation of the receptor for immunoglobulin E and its subunits in Triton X-114. Journal of Biological Chemistry. 1984;259(23):14922-14927.
Alcaraz, G. ; Kinet, J. P. ; Kumar, N. ; Wank, S. A. ; Metzger, H. / Phase separation of the receptor for immunoglobulin E and its subunits in Triton X-114. In: Journal of Biological Chemistry. 1984 ; Vol. 259, No. 23. pp. 14922-14927.
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