Phase separation in biology and disease—a symposium report

Jennifer Cable, Clifford Brangwynne, Geraldine Seydoux, David Cowburn, Rohit V. Pappu, Carlos A. Castañeda, Luke E. Berchowitz, Zhijuan Chen, Martin Jonikas, Abby Dernburg, Tanja Mittag, Nicolas L. Fawzi

Research output: Contribution to journalComment/debate

Abstract

Phase separation of multivalent protein and RNA molecules enables cells the formation of reversible nonstoichiometric, membraneless assemblies. These assemblies, referred to as biomolecular condensates, help with the spatial organization and compartmentalization of cellular matter. Each biomolecular condensate is defined by a distinct macromolecular composition. Distinct condensates have distinct preferential locations within cells, and they are associated with distinct biological functions, including DNA replication, RNA metabolism, signal transduction, synaptic transmission, and stress response. Several proteins found in biomolecular condensates have also been implicated in disease, including Huntington's disease, amyotrophic lateral sclerosis, and several types of cancer. Disease-associated mutations in these proteins have been found to affect the material properties of condensates as well as the driving forces for phase separation. Understanding the intrinsic and extrinsic forces driving the formation and dissolution of biomolecular condensates via spontaneous and driven phase separation is an important step in understanding the processes associated with biological regulation in health and disease.

Original languageEnglish (US)
JournalAnnals of the New York Academy of Sciences
DOIs
StatePublished - Jan 1 2019

Fingerprint

Phase separation
RNA
Proteins
Huntington Disease
Amyotrophic Lateral Sclerosis
DNA Replication
Synaptic Transmission
Signal transduction
Signal Transduction
Metabolism
Materials properties
Mutation
Dissolution
Health
Molecules
Symposium
DNA
Neoplasms
Chemical analysis
Protein

Keywords

  • biomolecular condensates
  • granules
  • membraneless organelles
  • phase diagram
  • phase separation
  • protein disorder

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Cable, J., Brangwynne, C., Seydoux, G., Cowburn, D., Pappu, R. V., Castañeda, C. A., ... Fawzi, N. L. (2019). Phase separation in biology and disease—a symposium report. Annals of the New York Academy of Sciences. https://doi.org/10.1111/nyas.14126

Phase separation in biology and disease—a symposium report. / Cable, Jennifer; Brangwynne, Clifford; Seydoux, Geraldine; Cowburn, David; Pappu, Rohit V.; Castañeda, Carlos A.; Berchowitz, Luke E.; Chen, Zhijuan; Jonikas, Martin; Dernburg, Abby; Mittag, Tanja; Fawzi, Nicolas L.

In: Annals of the New York Academy of Sciences, 01.01.2019.

Research output: Contribution to journalComment/debate

Cable, J, Brangwynne, C, Seydoux, G, Cowburn, D, Pappu, RV, Castañeda, CA, Berchowitz, LE, Chen, Z, Jonikas, M, Dernburg, A, Mittag, T & Fawzi, NL 2019, 'Phase separation in biology and disease—a symposium report', Annals of the New York Academy of Sciences. https://doi.org/10.1111/nyas.14126
Cable, Jennifer ; Brangwynne, Clifford ; Seydoux, Geraldine ; Cowburn, David ; Pappu, Rohit V. ; Castañeda, Carlos A. ; Berchowitz, Luke E. ; Chen, Zhijuan ; Jonikas, Martin ; Dernburg, Abby ; Mittag, Tanja ; Fawzi, Nicolas L. / Phase separation in biology and disease—a symposium report. In: Annals of the New York Academy of Sciences. 2019.
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