@article{66d63dc2a4f74ad58318a9324481621d,
title = "Phase I study of alvocidib followed by 7+3 (cytarabine + daunorubicin) in newly diagnosed acute myeloid leukemia",
abstract = "Purpose: Alvocidib is a cyclin-dependent kinase 9 inhibitor leading to downregulation of the antiapoptotic BCL-2 family member, MCL-1. Alvocidib has shown clinical activity in a timed sequential regimen with cytarabine and mitoxantrone in relapsed/refractory and newly diagnosed acute myeloid leukemia (AML) but has not been studied in combination with traditional 7{\th}3 induction therapy. Patients and Methods: A multiinstitutional phase I dose-escalation study of alvocidib on days 1–3 followed by 7{\th}3 (cytarabine 100 mg/m2/day i.v. infusion days 5–12 and daunorubicin 60 mg/m2 i.v. days 5–7) was performed in newly diagnosed AML ≤65 years. Core-binding factor AML was excluded. Results: There was no MTD on this study; the recommended phase II dose of alvocidib was 30 mg/m2 i.v. over 30 minutes followed by 60 mg/m2 i.v. infusion over 4 hours. There was one dose-limiting toxicity of cytokine release syndrome. The most common grade ≥3 nonhematologic toxicities were diarrhea (44%) and tumor lysis syndrome (34%). Overall, 69% (22/32) of patients achieved complete remission (CR). In an exploratory cohort, eight of nine (89%) patients in complete remission had no measurable residual disease, as determined by a centralized flow cytometric assay. Clinical activity was seen in patients with secondary AML, AML with myelodysplastic syndrome–related changes, and a genomic signature of secondary AML (50%, 50%, and 92% CR rates, respectively). Conclusions: Alvocidib can be safely administered prior to 7{\th}3 induction with encouraging clinical activity. These findings warrant further investigation of alvocidib combinations in newly diagnosed AML. This study was registered at clinicaltrials.gov identifier NCT03298984.",
author = "Zeidner, {Joshua F.} and Lee, {Daniel J.} and Mark Frattini and Fine, {Gil D.} and Judy Costas and Kathryn Kolibaba and Anthony, {Stephen P.} and David Bearss and {Douglas Smith}, B.",
note = "Funding Information: J.F. Zeidner reports grants from Tolero Pharmaceuticals (research funding) during the conduct of the study; personal fees from AbbVie (independent review committee), Agios (advisory board), Bristol Myers Squibb/Celgene (advisory board, consultancy), Daiichi-Sankyo (advisory board), Genentech (advisory board), Pfizer (advisory board), Takeda (advisory board, consultancy), Tolero Pharmaceuticals (advisory board), AsystBio Laboratories (consultancy); grants from AROG (research funding), Bristol Myers Squibb/Celgene (research funding), Forty Seven (research funding), Merck (research funding), Takeda (research funding), and Tolero Pharmaceuticals (research funding) outside the submitted work. D.J. Lee reports other from Tolero (institutional research funding) during the conduct of the study; personal fees from Celgene (consulting); other from AbbVie (institutional research funding), Bayer (institutional research funding), Forty Seven (institutional research funding), Genentech (institutional research funding), and Novartis (institutional research funding) outside the submitted work. M. Frattini reports personal fees from Celgene, Bristol-Myers Squibb, Lin BioSciences, and Cellectis outside the submitted work. K. Kolibaba reports personal fees and other from TG Therapeutics (consultancy, research funding); other from AbbVie (research funding), Acerta (research funding), Celgene (research funding), Cell Therapeutics (research funding), Genentech (research funding), Gilead (research funding), Janssen (research funding), Novartis (research funding), Pharmacyclics (research funding), and Seattle Genetics (research funding) outside the submitted work. S.P. Anthony reports personal fees from Exact Sciences (evidence review member) outside the submitted work; and work for Sumitomo Dainippon Pharma Oncology who is the sponsor of the trial. D. Bearss reports other from Sumitomo Dainippon Pharma Oncology fka Tolero Pharmaceuticals (employee and officer at this company) during the conduct of the study; in addition, D. Bearss has a patent for USPTO 10,682,356 pending to Tolero Pharmaceuticals (combination therapies for treatment of cancer), a patent for USPTO 10,624,880 pending to Tolero Pharmaceuticals (predicting response to alvocidib by mitochondrial profiling), a patent for USPTO 10,568,887 pending to Tolero Pharmaceuticals (combination therapies for treatment of cancer), a patent for USPTO 10,562,925 pending to Tolero Pharmaceuticals (alvocidib prodrugs having increased bioavailability), a patent for USPTO 10,422,788 pending to Tolero Pharmaceuticals (profiling peptides and methods for sensitivity profiling), a patent for USPTO 10,357,488 pending to Tolero Pharmaceuticals (predicting response to alvocidib by mitochondrial profiling), a patent for USPTO 10,267,787 pending to Tolero Pharmaceuticals (profiling peptides and methods for sensitivity profiling), a patent for USPTO 10,259,835 pending to Tolero Pharmaceuticals (alvocidib prodrugs having increased bioavailability), a patent for USPTO 10,132,797 pending to Tolero Pharmaceuticals (profiling peptides and methods for sensitivity profiling), a patent for USPTO 9,901,574 pending to Tolero Pharmaceuticals (predicting response to alvocidib by mitochondrial profiling), and a patent for USPTO 9,758,539 pending to Tolero Pharmaceuticals (alvocidib prodrugs having increased bioavailability); and Tolero Pharmaceuticals was funded by several individual and venture capital investors before its acquisition in 2017 by Sumitomo Dainippon Pharma, and aspects of the trial detailed in this published work were in process before and after this acquisition. Tolero Pharmaceuticals, as a single entity and then as a wholly owned subsidiary of Sumitomo Dainippon Pharma, funded the work described in this manuscript. B.D. Smith reports other from Jazz Pharma (consulting fees), Novartis (consulting fees), Pfizer (consulting fees), Celgene (DSMB), and Agios (consulting fees) outside the submitted work. No disclosures were reported by the other authors. Funding Information: Sonali Lokhande, MD, a medical writer from Criterion Edge supported by funding from Sumitomo Dainippon Pharma Oncology/Tolero Pharmaceuticals, provided editorial assistance to the authors during preparation of this manuscript. Publisher Copyright: {\textcopyright} 2020 American Association for Cancer Research.",
year = "2021",
month = jan,
day = "1",
doi = "10.1158/1078-0432.CCR-20-2649",
language = "English (US)",
volume = "27",
pages = "60--69",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "1",
}