Phase 1 trial of the Plasmodium falciparum blood stage vaccine MSP1 42-C1/alhydrogel with and without CPG 7909 in malaria naïve adults

Ruth D. Ellis, Laura B. Martin, Donna Shaffer, Carole A. Long, Kazutoyo Miura, Michael P. Fay, David L. Narum, Daming Zhu, Gregory E.D. Mullen, Siddhartha Mahanty, Louis H. Miller, Anna P. Durbin

Research output: Contribution to journalArticlepeer-review

Abstract

Background:Merozoite surface protein 142 (MSP142) is a leading blood stage malaria vaccine candidate. In order to induce immune responses that cover the major antigenic polymorphisms, FVO and 3D7 recombinant proteins of MSP142 were mixed (MSP142-C1). To improve the level of antibody response, MSP142-C1 was formulated with Alhydrogel plus the novel adjuvant CPG 7909. Methods:A Phase 1 clinical trial was conducted in healthy malaria-naïve adults at the Center for Immunization Research in Washington, D.C., to evaluate the safety and immunogenicity of MSP1 42-C1/Alhydrogel +/2 CPG 7909. Sixty volunteers were enrolled in dose escalating cohorts and randomized to receive three vaccinations of either 40 or 160 mg protein adsorbed to Alhydrogel +/2 560 μg CPG 7909 at 0, 1 and 2 months. Results: Vaccinations were well tolerated, with only one related adverse event graded as severe (Grade 3 injection site erythema) and all other vaccine related adverse events graded as either mild or moderate. Local adverse events were more frequent and severe in the groups receiving CPG. The addition of CPG enhanced anti-MSP142 antibody responses following vaccination by up to 49-fold two weeks after second immunization and 8-fold two weeks after the third immunization when compared to MSP142-C1/Alhydrogel alone (p<0.0001). After the third immunization, functionality of the antibody was tested by an in vitro growth inhibition assay. Inhibition was a function of antibody titer, with an average of 3% (range 22 to 10%) in the non CPG groups versus 14% (3 to 32%) in the CPG groups. Conclusion/Significance:The favorable safety profile and high antibody responses induced with MSP142-C1/ Alhydrogel + CPG 7909 are encouraging. MSP142-C1/Alhydrogel is being combined with other blood stage antigens and will be taken forward in a formulation adjuvanted with CPG 7909. Trial Registration: ClinicalTrials.gov Identifier: NCT00320658.

Original languageEnglish (US)
Article numbere8787
JournalPloS one
Volume5
Issue number1
DOIs
StatePublished - Jan 22 2010

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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