TY - JOUR
T1 - Pharmacological characterization of phosphoinositide-linked glutamate receptor excitation of hippocampal neurons
AU - Stratton, Kathleen R.
AU - Worley, Paul F.
AU - Baraban, Jay M.
N1 - Funding Information:
We thank D. Lawrence for excellent secretarial assistance. This work was supported by a PHS Grant DA-00266 (J.M.B.) and grants form the Lucille P. Markey Charitable Trust (J.M.B.), the Sloan Foundation (J.M.B.) and the Klingenstein Foundation (P.F.W.). J.M.B. is a Lucille P. Markey Scholar.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1990/9/21
Y1 - 1990/9/21
N2 - Pharmacological studies of glutamate receptor stimulation of the phosphoinositide (PI) system have demonstrated that this response is blocked by several agents: 2-amino-3-phosphonopropionate (AP3), phorbol esters and in some preparations pertussis toxin. In electrophysiological studies of CA1 pyramidal neurons, we have found that pertussis toxin and AP3 (1-2 mM) do not block either the membrane depolarization or inhibition of the slow afterhyperpolarization elicited by trans-1-aminocyclopentyl-1,3-dicarboxylate (ACPD; 30 μM), a selective agonist of the PI-linked glutamate receptor. However, phorbol 12,13-diacetate (1-1.5 μM) which itself blocks the slow afterhyperpolarization, completely blocks the membrane depolarizing response elicited by ACPD. These results add to growing evidence for heterogeneity among PI-linked glutamate receptor responses.
AB - Pharmacological studies of glutamate receptor stimulation of the phosphoinositide (PI) system have demonstrated that this response is blocked by several agents: 2-amino-3-phosphonopropionate (AP3), phorbol esters and in some preparations pertussis toxin. In electrophysiological studies of CA1 pyramidal neurons, we have found that pertussis toxin and AP3 (1-2 mM) do not block either the membrane depolarization or inhibition of the slow afterhyperpolarization elicited by trans-1-aminocyclopentyl-1,3-dicarboxylate (ACPD; 30 μM), a selective agonist of the PI-linked glutamate receptor. However, phorbol 12,13-diacetate (1-1.5 μM) which itself blocks the slow afterhyperpolarization, completely blocks the membrane depolarizing response elicited by ACPD. These results add to growing evidence for heterogeneity among PI-linked glutamate receptor responses.
KW - (Intracellular recording)
KW - Afterhyperpolarization
KW - Hippocampus
KW - Pertussis toxin
KW - Phorbol esters
KW - trans-ACPD (trans-1-aminocyclopentyl-1,3-dicarboxylate)
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U2 - 10.1016/0014-2999(90)90461-E
DO - 10.1016/0014-2999(90)90461-E
M3 - Article
C2 - 1963152
AN - SCOPUS:0025163880
VL - 186
SP - 357
EP - 361
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2-3
ER -