Abstract
Pharmacological studies of glutamate receptor stimulation of the phosphoinositide (PI) system have demonstrated that this response is blocked by several agents: 2-amino-3-phosphonopropionate (AP3), phorbol esters and in some preparations pertussis toxin. In electrophysiological studies of CA1 pyramidal neurons, we have found that pertussis toxin and AP3 (1-2 mM) do not block either the membrane depolarization or inhibition of the slow afterhyperpolarization elicited by trans-1-aminocyclopentyl-1,3-dicarboxylate (ACPD; 30 μM), a selective agonist of the PI-linked glutamate receptor. However, phorbol 12,13-diacetate (1-1.5 μM) which itself blocks the slow afterhyperpolarization, completely blocks the membrane depolarizing response elicited by ACPD. These results add to growing evidence for heterogeneity among PI-linked glutamate receptor responses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 357-361 |
| Number of pages | 5 |
| Journal | European Journal of Pharmacology |
| Volume | 186 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Sep 21 1990 |
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Keywords
- (Intracellular recording)
- Afterhyperpolarization
- Hippocampus
- Pertussis toxin
- Phorbol esters
- trans-ACPD (trans-1-aminocyclopentyl-1,3-dicarboxylate)
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Pharmacology
Cite this
Pharmacological characterization of phosphoinositide-linked glutamate receptor excitation of hippocampal neurons. / Stratton, Kathleen R.; Worley, Paul F; Baraban, Jay M.
In: European Journal of Pharmacology, Vol. 186, No. 2-3, 21.09.1990, p. 357-361.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pharmacological characterization of phosphoinositide-linked glutamate receptor excitation of hippocampal neurons
AU - Stratton, Kathleen R.
AU - Worley, Paul F
AU - Baraban, Jay M
PY - 1990/9/21
Y1 - 1990/9/21
N2 - Pharmacological studies of glutamate receptor stimulation of the phosphoinositide (PI) system have demonstrated that this response is blocked by several agents: 2-amino-3-phosphonopropionate (AP3), phorbol esters and in some preparations pertussis toxin. In electrophysiological studies of CA1 pyramidal neurons, we have found that pertussis toxin and AP3 (1-2 mM) do not block either the membrane depolarization or inhibition of the slow afterhyperpolarization elicited by trans-1-aminocyclopentyl-1,3-dicarboxylate (ACPD; 30 μM), a selective agonist of the PI-linked glutamate receptor. However, phorbol 12,13-diacetate (1-1.5 μM) which itself blocks the slow afterhyperpolarization, completely blocks the membrane depolarizing response elicited by ACPD. These results add to growing evidence for heterogeneity among PI-linked glutamate receptor responses.
AB - Pharmacological studies of glutamate receptor stimulation of the phosphoinositide (PI) system have demonstrated that this response is blocked by several agents: 2-amino-3-phosphonopropionate (AP3), phorbol esters and in some preparations pertussis toxin. In electrophysiological studies of CA1 pyramidal neurons, we have found that pertussis toxin and AP3 (1-2 mM) do not block either the membrane depolarization or inhibition of the slow afterhyperpolarization elicited by trans-1-aminocyclopentyl-1,3-dicarboxylate (ACPD; 30 μM), a selective agonist of the PI-linked glutamate receptor. However, phorbol 12,13-diacetate (1-1.5 μM) which itself blocks the slow afterhyperpolarization, completely blocks the membrane depolarizing response elicited by ACPD. These results add to growing evidence for heterogeneity among PI-linked glutamate receptor responses.
KW - (Intracellular recording)
KW - Afterhyperpolarization
KW - Hippocampus
KW - Pertussis toxin
KW - Phorbol esters
KW - trans-ACPD (trans-1-aminocyclopentyl-1,3-dicarboxylate)
UR - http://www.scopus.com/inward/record.url?scp=0025163880&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025163880&partnerID=8YFLogxK
U2 - 10.1016/0014-2999(90)90461-E
DO - 10.1016/0014-2999(90)90461-E
M3 - Article
C2 - 1963152
AN - SCOPUS:0025163880
VL - 186
SP - 357
EP - 361
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2-3
ER -