Pex13p is an SH3 protein of the peroxisome membrane and a docking factor for the predominantly cytoplasmic PTS1 receptor

Stephen J. Gould, Jennifer E. Kalish, James C. Morrell, Jonas Bjorkman, Aaron J. Urquhart, Denis I. Crane

Research output: Contribution to journalArticlepeer-review

Abstract

Import of newly synthesized PTS1 proteins into the peroxisome requires the PTS1 receptor (Pex5p), a predominantly cytoplasmic protein that cycles between the cytoplasm and peroxisome. We have identified Pex13p, a novel integral peroxisomal membrane from both yeast and humans that binds the PTS1 receptor via a cytoplasmically oriented SH3 domain. Although only a small amount of Pex5p is bound to peroxisomes at steady state (<5%), loss of Pex13p further reduces the amount of peroxisome-associated Pex5p by ~40-fold. Furthermore, loss of Pex13p eliminates import of peroxisomal matrix proteins that contain either the type-1 or type-2 peroxisomal targeting signal but does not affect targeting and insertion of integral peroxisomal membrane proteins. We conclude that Pex13p functions as a docking factor for the predominantly cytoplasmic PTS1 receptor.

Original languageEnglish (US)
Pages (from-to)85-95
Number of pages11
JournalJournal of Cell Biology
Volume135
Issue number1
DOIs
StatePublished - Oct 1996

ASJC Scopus subject areas

  • Cell Biology

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