Persistent propagation of variant creutzfeldt-jakob disease agent in murine spleen stromal cell culture with features of mesenchymal stem cells

Sergey Akimov, Oksana Yakovleva, Irina Vasilyeva, Carroll McKenzie, Larisa Cervenakova

Research output: Contribution to journalArticlepeer-review

Abstract

The transmission of variant Creutzfeldt-Jakob disease (vCJD) through blood transfusions has created new concerns about the iatrogenic spread of transmissible spongiform encephalopathies (TSEs)/prion diseases through blood and plasma-derived products and has increased the need to develop efficient methods for detection of the agent in biologics. Here, we report the first successful generation of spleen-derived murine stromal cell cultures that persistently propagate two mouse-adapted isolates of human TSE agents, mouseadapted vCJD, and Fukuoka 1. These new cell cultures can be used efficiently for studies of the pathogenesis of the disease, for development of diagnostics and therapeutics, and as a rapid ex vivo assay for TSE inactivation/removal procedures.

Original languageEnglish (US)
Pages (from-to)10959-10962
Number of pages4
JournalJournal of virology
Volume82
Issue number21
DOIs
StatePublished - Nov 2008
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Persistent propagation of variant creutzfeldt-jakob disease agent in murine spleen stromal cell culture with features of mesenchymal stem cells'. Together they form a unique fingerprint.

Cite this