Persistence of the interaction of calmodulin with adenylyl cyclase: Implications for integration of transient calcium stimuli

Chiadikaobi U Onyike, Allison H. Lin, Thomas W. Abrams

Research output: Contribution to journalArticle

Abstract

Ca2+/calmodulin-sensitive adenylyl cyclase plays a role in several forms of synaptic plasticity and learning. To understand how cellular signals from neuronal activity during behavioral stimuli might be integrated by adenylyl cyclase, we have characterized the response of type I adenylyl cyclase to transient Ca2+ stimuli. Stimulation by a several second Ca2+ stimulus is delayed, rising to a peak after the Ca2+ stimulus has ended. We attempted to identify the site of the persistent Ca2+ signal that enabled adenylyl cyclase stimulation to increase after free Ca2+ had declined. Free calmodulin itself displayed no persistent activation by Ca2+ and was unable to activate adenylyl cyclase if exposed to low Ca2+ solution 2+ stimulus. Activation decayed with a time constant of 6 or 13 s depending on assay conditions. These results suggest that the calmodulin-adenylyl cyclase complex can serve as a site of cellular memory for a Ca2+ transient that has ended even before adenylyl cyclase is fully activated.

Original languageEnglish (US)
Pages (from-to)1298-1306
Number of pages9
JournalJournal of Neurochemistry
Volume71
Issue number3
StatePublished - Sep 1998
Externally publishedYes

Fingerprint

Calmodulin
Adenylyl Cyclases
Calcium
Chemical activation
Neuronal Plasticity
Plasticity
Assays
Learning
Data storage equipment

Keywords

  • Adenylyl cyclase
  • Associative learning
  • Ca
  • Calmodulin
  • Coincidence detector
  • Synaptic plasticity
  • Transient Ca stimuli

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Persistence of the interaction of calmodulin with adenylyl cyclase : Implications for integration of transient calcium stimuli. / Onyike, Chiadikaobi U; Lin, Allison H.; Abrams, Thomas W.

In: Journal of Neurochemistry, Vol. 71, No. 3, 09.1998, p. 1298-1306.

Research output: Contribution to journalArticle

@article{3cce6ad2fce14b7b81c4735c35925573,
title = "Persistence of the interaction of calmodulin with adenylyl cyclase: Implications for integration of transient calcium stimuli",
abstract = "Ca2+/calmodulin-sensitive adenylyl cyclase plays a role in several forms of synaptic plasticity and learning. To understand how cellular signals from neuronal activity during behavioral stimuli might be integrated by adenylyl cyclase, we have characterized the response of type I adenylyl cyclase to transient Ca2+ stimuli. Stimulation by a several second Ca2+ stimulus is delayed, rising to a peak after the Ca2+ stimulus has ended. We attempted to identify the site of the persistent Ca2+ signal that enabled adenylyl cyclase stimulation to increase after free Ca2+ had declined. Free calmodulin itself displayed no persistent activation by Ca2+ and was unable to activate adenylyl cyclase if exposed to low Ca2+ solution 2+ stimulus. Activation decayed with a time constant of 6 or 13 s depending on assay conditions. These results suggest that the calmodulin-adenylyl cyclase complex can serve as a site of cellular memory for a Ca2+ transient that has ended even before adenylyl cyclase is fully activated.",
keywords = "Adenylyl cyclase, Associative learning, Ca, Calmodulin, Coincidence detector, Synaptic plasticity, Transient Ca stimuli",
author = "Onyike, {Chiadikaobi U} and Lin, {Allison H.} and Abrams, {Thomas W.}",
year = "1998",
month = "9",
language = "English (US)",
volume = "71",
pages = "1298--1306",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Persistence of the interaction of calmodulin with adenylyl cyclase

T2 - Implications for integration of transient calcium stimuli

AU - Onyike, Chiadikaobi U

AU - Lin, Allison H.

AU - Abrams, Thomas W.

PY - 1998/9

Y1 - 1998/9

N2 - Ca2+/calmodulin-sensitive adenylyl cyclase plays a role in several forms of synaptic plasticity and learning. To understand how cellular signals from neuronal activity during behavioral stimuli might be integrated by adenylyl cyclase, we have characterized the response of type I adenylyl cyclase to transient Ca2+ stimuli. Stimulation by a several second Ca2+ stimulus is delayed, rising to a peak after the Ca2+ stimulus has ended. We attempted to identify the site of the persistent Ca2+ signal that enabled adenylyl cyclase stimulation to increase after free Ca2+ had declined. Free calmodulin itself displayed no persistent activation by Ca2+ and was unable to activate adenylyl cyclase if exposed to low Ca2+ solution 2+ stimulus. Activation decayed with a time constant of 6 or 13 s depending on assay conditions. These results suggest that the calmodulin-adenylyl cyclase complex can serve as a site of cellular memory for a Ca2+ transient that has ended even before adenylyl cyclase is fully activated.

AB - Ca2+/calmodulin-sensitive adenylyl cyclase plays a role in several forms of synaptic plasticity and learning. To understand how cellular signals from neuronal activity during behavioral stimuli might be integrated by adenylyl cyclase, we have characterized the response of type I adenylyl cyclase to transient Ca2+ stimuli. Stimulation by a several second Ca2+ stimulus is delayed, rising to a peak after the Ca2+ stimulus has ended. We attempted to identify the site of the persistent Ca2+ signal that enabled adenylyl cyclase stimulation to increase after free Ca2+ had declined. Free calmodulin itself displayed no persistent activation by Ca2+ and was unable to activate adenylyl cyclase if exposed to low Ca2+ solution 2+ stimulus. Activation decayed with a time constant of 6 or 13 s depending on assay conditions. These results suggest that the calmodulin-adenylyl cyclase complex can serve as a site of cellular memory for a Ca2+ transient that has ended even before adenylyl cyclase is fully activated.

KW - Adenylyl cyclase

KW - Associative learning

KW - Ca

KW - Calmodulin

KW - Coincidence detector

KW - Synaptic plasticity

KW - Transient Ca stimuli

UR - http://www.scopus.com/inward/record.url?scp=0031844212&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031844212&partnerID=8YFLogxK

M3 - Article

C2 - 9721756

AN - SCOPUS:0031844212

VL - 71

SP - 1298

EP - 1306

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 3

ER -