Persistence of pulmonary pathology and abnormal lung function in IL-3/GM-CSF/IL-5 βc receptor-deficient mice despite correction of alveolar proteinosis after BMT

Kenneth R Cooke, R. Nishinakamura, T. R. Martin, L. Kobzik, J. Brewer, J. A. Whitsett, D. Bungard, R. Murray, J. L M Ferrara

Research output: Contribution to journalArticle

Abstract

Mice deficient for the IL-3/GM-CSF/IL-5 βc receptor (βcR KO) develop lung disease similar to that seen in human pulmonary alveolar proteinosis (PAP) which includes lymphocytic infiltration around airways and vessels and the progressive accumulation of surfactant and macrophages within the alveolar space. We investigated bone marrow transplantation (BMT) as a curative treatment of PAP in βcR KO mice by semiquantitative histologic analysis and evaluation of pulmonary function, BMT from wild-type (WT) donors into lethally irradiated βcR KO recipients (WT → KO) led to the complete resolution of alveolar protein accumulation and to normalization of BAL fluid cellularity and macrophage morphology. However, detailed microscopic analysis of lung tissue revealed the persistence of significant cellular infiltrates in WT → KO recipients which were equivalent to those seen in KO → KO animals. Evaluation of pulmonary function demonstrated that only dynamic compliance (C(dyn)) and not airway conductance (G(L)) was significantly improved in the WT → KO group compared to KO → KO animals and that both of these measurements remained significantly abnormal when compared to WT → WT controls. We conclude, that although BMT for PAP reverses alveolar macrophage and protein accumulation, it does not decrease the interstitial inflammatory component of this disease. The importance of this residual pathology is demonstrated by the incomplete correction of alveolar function (C(dyn)) and lack of improvement in increased airway resistance (G(L)). These findings may have important implications with regard to the extent that BMT can be considered a potential curative procedure for this clinical disorder.

Original languageEnglish (US)
Pages (from-to)657-662
Number of pages6
JournalBone Marrow Transplantation
Volume20
Issue number8
StatePublished - Oct 2 1997
Externally publishedYes

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Interleukin-5 Receptors
Interleukin-3
Pulmonary Alveolar Proteinosis
Granulocyte-Macrophage Colony-Stimulating Factor
Bone Marrow Transplantation
Pathology
Lung
Alveolar Macrophages
Dimercaprol
Airway Resistance
Surface-Active Agents
Lung Diseases
Compliance
Proteins
Macrophages

Keywords

  • βc deficient
  • BMT
  • Lung
  • Pathology
  • PFTs

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Persistence of pulmonary pathology and abnormal lung function in IL-3/GM-CSF/IL-5 βc receptor-deficient mice despite correction of alveolar proteinosis after BMT. / Cooke, Kenneth R; Nishinakamura, R.; Martin, T. R.; Kobzik, L.; Brewer, J.; Whitsett, J. A.; Bungard, D.; Murray, R.; Ferrara, J. L M.

In: Bone Marrow Transplantation, Vol. 20, No. 8, 02.10.1997, p. 657-662.

Research output: Contribution to journalArticle

Cooke, KR, Nishinakamura, R, Martin, TR, Kobzik, L, Brewer, J, Whitsett, JA, Bungard, D, Murray, R & Ferrara, JLM 1997, 'Persistence of pulmonary pathology and abnormal lung function in IL-3/GM-CSF/IL-5 βc receptor-deficient mice despite correction of alveolar proteinosis after BMT', Bone Marrow Transplantation, vol. 20, no. 8, pp. 657-662.
Cooke, Kenneth R ; Nishinakamura, R. ; Martin, T. R. ; Kobzik, L. ; Brewer, J. ; Whitsett, J. A. ; Bungard, D. ; Murray, R. ; Ferrara, J. L M. / Persistence of pulmonary pathology and abnormal lung function in IL-3/GM-CSF/IL-5 βc receptor-deficient mice despite correction of alveolar proteinosis after BMT. In: Bone Marrow Transplantation. 1997 ; Vol. 20, No. 8. pp. 657-662.
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