Persistence length changes dramatically as RNA folds

G. Caliskan, C. Hyeon, U. Perez-Salas, R. M. Briber, S. A. Woodson, D. Thirumalai

Research output: Contribution to journalArticle

Abstract

We determine the persistence length lp for a bacterial group I ribozyme as a function of concentration of monovalent and divalent cations by fitting the distance distribution functions P(r) obtained from small angle x-ray scattering intensity data to the asymptotic form of the calculated PWLC(r) for a wormlike chain. The lp values change dramatically over a narrow range of Mg2+ concentration from ∼21 in the unfolded state (U) to ∼10 in the compact (IC) and native states. Variations in lp with increasing Na+ concentration are more gradual. In accord with the predictions of polyelectrolyte theory we find lp1/κ2 where κ is the inverse Debye-screening length.

Original languageEnglish (US)
Article number268303
JournalPhysical Review Letters
Volume95
Issue number26
DOIs
StatePublished - Dec 31 2005

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x ray scattering
screening
distribution functions
cations
predictions

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Caliskan, G., Hyeon, C., Perez-Salas, U., Briber, R. M., Woodson, S. A., & Thirumalai, D. (2005). Persistence length changes dramatically as RNA folds. Physical Review Letters, 95(26), [268303]. https://doi.org/10.1103/PhysRevLett.95.268303

Persistence length changes dramatically as RNA folds. / Caliskan, G.; Hyeon, C.; Perez-Salas, U.; Briber, R. M.; Woodson, S. A.; Thirumalai, D.

In: Physical Review Letters, Vol. 95, No. 26, 268303, 31.12.2005.

Research output: Contribution to journalArticle

Caliskan, G, Hyeon, C, Perez-Salas, U, Briber, RM, Woodson, SA & Thirumalai, D 2005, 'Persistence length changes dramatically as RNA folds', Physical Review Letters, vol. 95, no. 26, 268303. https://doi.org/10.1103/PhysRevLett.95.268303
Caliskan G, Hyeon C, Perez-Salas U, Briber RM, Woodson SA, Thirumalai D. Persistence length changes dramatically as RNA folds. Physical Review Letters. 2005 Dec 31;95(26). 268303. https://doi.org/10.1103/PhysRevLett.95.268303
Caliskan, G. ; Hyeon, C. ; Perez-Salas, U. ; Briber, R. M. ; Woodson, S. A. ; Thirumalai, D. / Persistence length changes dramatically as RNA folds. In: Physical Review Letters. 2005 ; Vol. 95, No. 26.
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