Peroxynitrite inhibition of coxsackievirus infection by prevention of viral RNA entry

Elizaveta Padalko, Tomokazu Ohnishi, Kenji Matsushita, Henry Sun, Karen Fox-Talbot, Clare Bao, William M. Baldwin, Charles J. Lowenstein

Research output: Contribution to journalArticlepeer-review

Abstract

Although peroxynitrite is harmful to the host, the beneficial effects of peroxynitrite are less well understood. We explored the role of peroxynitrite in the host immune response to Coxsackievirus infection. Peroxynitrite inhibits viral replication in vitro, in part by inhibiting viral RNA entry into the host cell. Nitrotyrosine, a marker for peroxynitrite production, is colocalized with viral antigens in the hearts of infected mice but not control mice. Nitrotyrosine coprecipitates with the viral polypeptide VP1 as well. Guanidinoethyl disulfide, a scavenger of peroxynitrite, blocks peroxynitrite inhibition of viral replication in vitro and permits an increase in viral replication in vivo. These data suggest that peroxynitrite is an endogenous effector of the immune response to viruses.

Original languageEnglish (US)
Pages (from-to)11731-11736
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number32
DOIs
StatePublished - Aug 10 2004

Keywords

  • Nitric oxide
  • Nitrotyrosine
  • Picornavirus
  • Superoxide
  • Virus

ASJC Scopus subject areas

  • General

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