Peroxynitrite does not impair pulmonary and systemic vascular responses

B. D. Nossaman, P. A. Dabisch, J. T. Liles, S. R. Baber, H. C. Champion, A. D. Kaye, C. J. Feng, M. Anwar, T. J. Bivalacqua, J. A. Santiago, B. J. De Witt, P. J. Kadowitz

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of peroxynitrite (ONOO-) on vascular responses were investigated in the systemic and hindquarters vascular bed and in the isolated perfused rat lung. Intravenous injections of ONOO- decreased systemic arterial pressure, and injections of ONOO- into the hindquarters decreased perfusion pressure in a dose-related manner. Injections of ONOO- into the lung perfusion circuit increased pulmonary arterial perfusion pressure. Responses to ONOO- were rapid in onset, short in duration, and repeatable without exhibiting tachyphylaxis. Repeated injections of ONOO- did not alter systemic, hindquarters, or pulmonary responses to endothelium-dependent vasodilators or other vasoactive agonists and did not alter the hypoxic pulmonary vasoconstrictor response. Injections of sodium nitrate or nitrite or decomposed ONOO- had little effect on vascular pressures. Pulmonary and hindquarters responses to ONOO- were not altered by a cyclooxygenase inhibitor in a dose that attenuated responses to arachidonic acid. These results demonstrate that ONOO- has significant pulmonary vasoconstrictor, systemic vasodepressor, and vasodilator activity; that short-term repeated exposure does impair vascular responsiveness; and that responses to ONOO- are not dependent on cyclooxygenase product release.

Original languageEnglish (US)
Pages (from-to)455-462
Number of pages8
JournalJournal of applied physiology
Volume96
Issue number2
DOIs
StatePublished - Feb 2004
Externally publishedYes

Keywords

  • Cyclooxygenase product
  • Hypoxia
  • Oxidant
  • Vasoconstrictor responses
  • Vasodilator responses

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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