Peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1) plays a critical role in regulating multiple aspects of energy metabolism, including adaptive thermogenesis, mitochondrial biogenesis, and fatty acid β-oxidation. Recently, this coactivator of nuclear receptors/transcription factors has been shown to control hepatic gluconeogenesis, an important component of the pathogenesis of both type-1 and type-2 diabetes. We described here the cloning of a novel bona fide homologue of PGC-1, PGC-1β (PGC-1 was renamed as PGC-1α), first identified through searches of new data base entries. Despite the fact that PGC-1α and -1β share similar tissue distributions with highest levels of expression in brown fat and heart, their mRNAs are differentially regulated in the brown adipose tissue upon cold exposure and during brown fat cell differentiation. Like PGC-1α, PGC-1β mRNA levels are increased significantly in the liver during fasting, suggesting a possible role for this factor in the regulation of hepatic gluconeogenesis and/or fatty acid oxidation. Consistent with this, PGC-1β was shown to physically interact and potently coactivate hepatic nuclear factor 4 and peroxisome proliferator-activated receptor α, nuclear receptors that are essential for hepatic adaptation to fasting. Finally, using sequence comparisons between PGC-1α and -1β, we have identified a conserved amino acid motif that serves as a docking site for host cell factor, a cellular protein implicated in cell cycle regulation and viral infection. HCF is shown to bind to both PGC-1α and -1β and augment their transcriptional activity.
ASJC Scopus subject areas