Peripolesis in alveolar sarcoidosis

T. C. Van Maarsseveen, J. De Groot, J. Stam, P. J. Van Diest

Research output: Contribution to journalArticle

Abstract

We observed that in bronchoalveolar lavages (BAL) of patients with active sarcoidosis (SARC) a mononuclear cell infiltrate is present that often contains clusters consisting of lymphocytes adhering to a macrophage. In order to investigate what kind of cellular interactions are involved in such a process, cell suspensions obtained from BAL of patients with SARC or extrinsic allergic alveolitis (EAA) were cultured for 1 to 2 days, during which time lapse cinematography was applied. We were able to show that such clusters consist of lymphocytes gathered around a macrophage. This is known as peripolesis. Peripolesis, as observed in our BAL, could last for some minutes or for some hours during which time a number of lymphocytes were moving around a single alveolar macrophage, without losing contact with the macrophage. Short interactions were mostly observed in EAA, whereas SARC was characterized by long periods of lymphocyte-macrophage cooperation. We also found a correlation between the time-dependent peripolesis t > 30 min/t <30 min and the CD4/CD8 ratio. Although the precise mechanisms of peripolesis are not well understood, some interactions between lymphocytes and macrophages have now become more comprehensive.

Original languageEnglish (US)
Pages (from-to)1259-1263
Number of pages5
JournalAmerican Review of Respiratory Disease
Volume147
Issue number5
StatePublished - 1993
Externally publishedYes

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Sarcoidosis
Macrophages
Bronchoalveolar Lavage
Extrinsic Allergic Alveolitis
Lymphocytes
Lymphocyte Cooperation
Cineradiography
CD4-CD8 Ratio
Alveolar Macrophages
Lymphocyte Count
Suspensions

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Van Maarsseveen, T. C., De Groot, J., Stam, J., & Van Diest, P. J. (1993). Peripolesis in alveolar sarcoidosis. American Review of Respiratory Disease, 147(5), 1259-1263.

Peripolesis in alveolar sarcoidosis. / Van Maarsseveen, T. C.; De Groot, J.; Stam, J.; Van Diest, P. J.

In: American Review of Respiratory Disease, Vol. 147, No. 5, 1993, p. 1259-1263.

Research output: Contribution to journalArticle

Van Maarsseveen, TC, De Groot, J, Stam, J & Van Diest, PJ 1993, 'Peripolesis in alveolar sarcoidosis', American Review of Respiratory Disease, vol. 147, no. 5, pp. 1259-1263.
Van Maarsseveen TC, De Groot J, Stam J, Van Diest PJ. Peripolesis in alveolar sarcoidosis. American Review of Respiratory Disease. 1993;147(5):1259-1263.
Van Maarsseveen, T. C. ; De Groot, J. ; Stam, J. ; Van Diest, P. J. / Peripolesis in alveolar sarcoidosis. In: American Review of Respiratory Disease. 1993 ; Vol. 147, No. 5. pp. 1259-1263.
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