TY - JOUR
T1 - Periplakin and envoplakin are target antigens in canine and human paraneoplastic pemphigus
AU - De Bruin, A.
AU - Muller, E.
AU - Wyder, M.
AU - Anhalt, G. J.
AU - Lemmens, P.
AU - Suter, M. M.
N1 - Funding Information:
Supported by the Swiss National Foundation (31-50855.SF) for Scientific Research.
PY - 1999
Y1 - 1999
N2 - Background: On the basis of clinical and histopathologic similarities to human paraneoplastic pemphigus (PNP), we recently identified the first case of PNP in a nonhuman species, the dog. Objective: To determine a similar pathogenesis in both species, the present study aimed to define whether common antigens are targeted in dog and man. Methods: Canine and human PNP sera were used in parallel to immunoprecipitate 14C-labeled human keratinocyte antigens. The immunoreactive proteins were then identified by immunoprecipitation of canine keratinocyte extracts with specific antibodies to the antiplakin family members followed by immunoblot analysis using canine and human PNP sera. Results: Protein bands of 210, 190, 170, and 130 kd were identified in dogs and humans. In both species, envoplakin and periplakin were demonstrated as antigens. Antidesmoglein 3 antibodies could not be demonstrated in canine PNP, but in human PNP. Conclusion: These results demonstrate that canine PNP closely correlates to the human counterpart and may therefore represent an excellent model for the human disease.
AB - Background: On the basis of clinical and histopathologic similarities to human paraneoplastic pemphigus (PNP), we recently identified the first case of PNP in a nonhuman species, the dog. Objective: To determine a similar pathogenesis in both species, the present study aimed to define whether common antigens are targeted in dog and man. Methods: Canine and human PNP sera were used in parallel to immunoprecipitate 14C-labeled human keratinocyte antigens. The immunoreactive proteins were then identified by immunoprecipitation of canine keratinocyte extracts with specific antibodies to the antiplakin family members followed by immunoblot analysis using canine and human PNP sera. Results: Protein bands of 210, 190, 170, and 130 kd were identified in dogs and humans. In both species, envoplakin and periplakin were demonstrated as antigens. Antidesmoglein 3 antibodies could not be demonstrated in canine PNP, but in human PNP. Conclusion: These results demonstrate that canine PNP closely correlates to the human counterpart and may therefore represent an excellent model for the human disease.
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U2 - 10.1016/S0190-9622(99)70147-7
DO - 10.1016/S0190-9622(99)70147-7
M3 - Article
C2 - 10321593
AN - SCOPUS:0032894756
SN - 0190-9622
VL - 40
SP - 682
EP - 685
JO - Journal of the American Academy of Dermatology
JF - Journal of the American Academy of Dermatology
IS - 5 I
ER -