Peripheral-type benzodiazepine receptors in endocrine organs: Autoradiographic localization in rat pituitary, adrenal, and testis

Errol B. De Souza, Robert R.H. Anholt, Kenneth M.M. Murphy, Solomon H. Snyder, Michael J. Kuhar

Research output: Contribution to journalArticlepeer-review

Abstract

We have used [3H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, to identify and localize peripheral-type benzodiazepine receptors in endocrine organs. Autoradiographic studies reveal an uniform distribution of [3H]Ro5-4864 binding sites within the anterior, intermediate, and posterior lobes of the pituitary gland, with highest concentrations present in the posterior pituitary. In rat adrenal gland, specific binding sites for [3H]Ro5-4864 are found only in the adrenal cortex, with highest density in the zona glomerulosa and significantly lower concentrations in the zona fasciculata and zona reticularis. [3H]Ro5-4864-associated silver grains in the testis are intensely localized over the interstitial tissue; low concentrations of silver grains are present over the epithelium of the seminiferous tubules but are absent from the tubular lumen. These studies demonstrate a differential and discrete localization of peripheral-type benzodiazepine receptors in rat pituitary, adrenal, and testis.

Original languageEnglish (US)
Pages (from-to)567-573
Number of pages7
JournalEndocrinology
Volume116
Issue number2
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Endocrinology

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