Peripheral regulation of graft-versus-host alloreactivity in mixed chimeric miniature swine

Shaun Kunisaki, Gary W. Haller, Yasushi Fuchimoto, Christene A. Huang, David H. Sachs, G. Tenderich

Research output: Contribution to journalArticle

Abstract

Background. Despite the presence of circulating donor-derived T cells during the induction of mixed chimerism across MHC barriers in miniature swine, severe graft-versus-host disease was avoided in the majority of animals. In this study, we investigated the possible roles of recipient and donor lymphoid populations in the regulation of donor-anti-recipient alloreactivity. Methods. Mixed chimerism across a full MHC-mismatch barrier was established in miniature swine using a high-dose allogeneic peripheral blood stem cell protocol. Peripheral blood mononuclear cells from mixed chimeric swine were co-cultured with naïve donor-matched responders and naive recipient-matched stimulators in mixed lymphocyte reactions. Results. Peripheral blood mononuclear cells from mixed chimeras inhibited donor-anti-recipient proliferation. This suppression was radioresistant to 25 Gy. Suppression of donor-anti-recipient alloreactivity was not observed in mixed lymphocyte co-cultures when donor-derived cells were added in the absence of recipient-derived cells. Conclusions. These results suggest an association between the presence of an active and relatively radioresistant cell population, demonstrable in vitro, and the regulation of graft-versus-host disease across MHC barriers in mixed chimeric miniature swine.

Original languageEnglish (US)
Pages (from-to)523-526
Number of pages4
JournalTransplantation
Volume72
Issue number3
DOIs
StatePublished - Aug 15 2001
Externally publishedYes

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Miniature Swine
Chimerism
Graft vs Host Disease
Transplants
Blood Cells
Mixed Lymphocyte Culture Test
Coculture Techniques
Population
Swine
Lymphocytes
T-Lymphocytes

ASJC Scopus subject areas

  • Transplantation

Cite this

Peripheral regulation of graft-versus-host alloreactivity in mixed chimeric miniature swine. / Kunisaki, Shaun; Haller, Gary W.; Fuchimoto, Yasushi; Huang, Christene A.; Sachs, David H.; Tenderich, G.

In: Transplantation, Vol. 72, No. 3, 15.08.2001, p. 523-526.

Research output: Contribution to journalArticle

Kunisaki, Shaun ; Haller, Gary W. ; Fuchimoto, Yasushi ; Huang, Christene A. ; Sachs, David H. ; Tenderich, G. / Peripheral regulation of graft-versus-host alloreactivity in mixed chimeric miniature swine. In: Transplantation. 2001 ; Vol. 72, No. 3. pp. 523-526.
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