Peripheral murine CD3+, CD4-, CD8- T lymphocytes express novel T cell receptor γδ structures

R. Q. Cron, F. Koning, W. L. Maloy, Andrew Mark Pardoll, J. E. Coligan, J. A. Bluestone

Research output: Contribution to journalArticle

Abstract

A mAb directed against the CD3 molecule was used to identify a subset of CD3+, CD4-, CD8- T cells previously undefined in the peripheral lymphoid organs of the mouse. Biochemical analysis of CFD3+, CD4-, CD8- splenocytes revealed that the vast majority of these cells express one of at least two distinct CD3-associated TCR γδ heterodimeric structures, but no detectable TCRαβ. One disulfide-linked heterodimer (77 kDa) is composed of two chains of 45 to 46 and 32 kDa. The latter chain was immunoprecipitated with an anti-TCR C(γ)1/C(γ)2 antiserum and was not glycosylated. An antiserum produced against a peptide corresponding to the C-terminal region of the predicted C(γ)4 gene product immunoprecipitated additional heterodimers (80 to 90 kDa). One heterodimer, composed of disulfide-linked 41- to 45-kDa proteins (including a V(γ)/C(γ)4 component), is expressed on a T cell hybridoma, DN-1.21, which was derived from fused splenic CD3+, CD4-, CD8- T cells. Another V(γ)/C(γ)4-containing heterodimer is composed of disulfide-linked 46- to 47-kDa glycoproteins. These findings demonstrate that CD3+, CD4-, CD8- T cells present in the peripheral lymphoid organs express a variety of paired TCRγδ proteins. Unlike CD3+, CD4-, CD8- thymocytes, these cells express high levels of C(γ)4, but little, if any TCRαβ.

Original languageEnglish (US)
Pages (from-to)1074-1082
Number of pages9
JournalJournal of Immunology
Volume141
Issue number4
StatePublished - 1988

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T-Cell Antigen Receptor
Disulfides
T-Lymphocytes
Immune Sera
Hybridomas
Thymocytes
Glycoproteins
Proteins
Peptides
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Cron, R. Q., Koning, F., Maloy, W. L., Pardoll, A. M., Coligan, J. E., & Bluestone, J. A. (1988). Peripheral murine CD3+, CD4-, CD8- T lymphocytes express novel T cell receptor γδ structures. Journal of Immunology, 141(4), 1074-1082.

Peripheral murine CD3+, CD4-, CD8- T lymphocytes express novel T cell receptor γδ structures. / Cron, R. Q.; Koning, F.; Maloy, W. L.; Pardoll, Andrew Mark; Coligan, J. E.; Bluestone, J. A.

In: Journal of Immunology, Vol. 141, No. 4, 1988, p. 1074-1082.

Research output: Contribution to journalArticle

Cron, RQ, Koning, F, Maloy, WL, Pardoll, AM, Coligan, JE & Bluestone, JA 1988, 'Peripheral murine CD3+, CD4-, CD8- T lymphocytes express novel T cell receptor γδ structures', Journal of Immunology, vol. 141, no. 4, pp. 1074-1082.
Cron, R. Q. ; Koning, F. ; Maloy, W. L. ; Pardoll, Andrew Mark ; Coligan, J. E. ; Bluestone, J. A. / Peripheral murine CD3+, CD4-, CD8- T lymphocytes express novel T cell receptor γδ structures. In: Journal of Immunology. 1988 ; Vol. 141, No. 4. pp. 1074-1082.
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