Peripheral blood mononuclear cells during natural measles virus infection: Cell surface phenotypes and evidence for activation

Diane Griffin, Thomas R. Moench, Richard Johnson, Imelda Lindo de Soriano, Abraham Vaisberg

Research output: Contribution to journalArticle

Abstract

Peripheral blood mononuclear cells from 25 patients with measles and 13 patients with other diseases from Lima, Peru, were studied by immunocytochemical staining for cell surface antigens indicating the type of cell (Leu 4, Leu 3, T8, B1, M1, or esterase) and the state of cell activation (T10 and IL-2 receptor). Measles patients were studied during the first 2 weeks of disease and had no alteration in the proportion of cells which were positive for any subset marker or in the ratio of helper/inducer to cytotoxic/suppressor T cells compared to controls. Measles patients, however, had a greater number of cells expressing the activation antigens T10 and IL-2 receptor than controls. Incorporation of [3H]thymidine was also higher in measles patients when exogenous natural or recombinant IL-2 was added to unstimulated cultured cells. We conclude that the peripheral blood mononuclear leukocytes of patients with measles have normal proportions of helper/inducer and cytotoxic/suppressor T lymphocytes, B lymphocytes, and monocytes but that an increased number of these cells are in an activated state.

Original languageEnglish (US)
Pages (from-to)305-312
Number of pages8
JournalClinical Immunology and Immunopathology
Volume40
Issue number2
DOIs
StatePublished - 1986

Fingerprint

Measles virus
Virus Diseases
Measles
Blood Cells
Phenotype
Interleukin-2 Receptors
CD38 Antigens
Cell Count
Mononuclear Leukocytes
Peru
Cytotoxic T-Lymphocytes
Esterases
Surface Antigens
Thymidine
Interleukin-2
Monocytes
Cultured Cells
B-Lymphocytes
Staining and Labeling
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Peripheral blood mononuclear cells during natural measles virus infection : Cell surface phenotypes and evidence for activation. / Griffin, Diane; Moench, Thomas R.; Johnson, Richard; de Soriano, Imelda Lindo; Vaisberg, Abraham.

In: Clinical Immunology and Immunopathology, Vol. 40, No. 2, 1986, p. 305-312.

Research output: Contribution to journalArticle

@article{75313304b41040fa994d15765be40ba5,
title = "Peripheral blood mononuclear cells during natural measles virus infection: Cell surface phenotypes and evidence for activation",
abstract = "Peripheral blood mononuclear cells from 25 patients with measles and 13 patients with other diseases from Lima, Peru, were studied by immunocytochemical staining for cell surface antigens indicating the type of cell (Leu 4, Leu 3, T8, B1, M1, or esterase) and the state of cell activation (T10 and IL-2 receptor). Measles patients were studied during the first 2 weeks of disease and had no alteration in the proportion of cells which were positive for any subset marker or in the ratio of helper/inducer to cytotoxic/suppressor T cells compared to controls. Measles patients, however, had a greater number of cells expressing the activation antigens T10 and IL-2 receptor than controls. Incorporation of [3H]thymidine was also higher in measles patients when exogenous natural or recombinant IL-2 was added to unstimulated cultured cells. We conclude that the peripheral blood mononuclear leukocytes of patients with measles have normal proportions of helper/inducer and cytotoxic/suppressor T lymphocytes, B lymphocytes, and monocytes but that an increased number of these cells are in an activated state.",
author = "Diane Griffin and Moench, {Thomas R.} and Richard Johnson and {de Soriano}, {Imelda Lindo} and Abraham Vaisberg",
year = "1986",
doi = "10.1016/0090-1229(86)90035-8",
language = "English (US)",
volume = "40",
pages = "305--312",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Peripheral blood mononuclear cells during natural measles virus infection

T2 - Cell surface phenotypes and evidence for activation

AU - Griffin, Diane

AU - Moench, Thomas R.

AU - Johnson, Richard

AU - de Soriano, Imelda Lindo

AU - Vaisberg, Abraham

PY - 1986

Y1 - 1986

N2 - Peripheral blood mononuclear cells from 25 patients with measles and 13 patients with other diseases from Lima, Peru, were studied by immunocytochemical staining for cell surface antigens indicating the type of cell (Leu 4, Leu 3, T8, B1, M1, or esterase) and the state of cell activation (T10 and IL-2 receptor). Measles patients were studied during the first 2 weeks of disease and had no alteration in the proportion of cells which were positive for any subset marker or in the ratio of helper/inducer to cytotoxic/suppressor T cells compared to controls. Measles patients, however, had a greater number of cells expressing the activation antigens T10 and IL-2 receptor than controls. Incorporation of [3H]thymidine was also higher in measles patients when exogenous natural or recombinant IL-2 was added to unstimulated cultured cells. We conclude that the peripheral blood mononuclear leukocytes of patients with measles have normal proportions of helper/inducer and cytotoxic/suppressor T lymphocytes, B lymphocytes, and monocytes but that an increased number of these cells are in an activated state.

AB - Peripheral blood mononuclear cells from 25 patients with measles and 13 patients with other diseases from Lima, Peru, were studied by immunocytochemical staining for cell surface antigens indicating the type of cell (Leu 4, Leu 3, T8, B1, M1, or esterase) and the state of cell activation (T10 and IL-2 receptor). Measles patients were studied during the first 2 weeks of disease and had no alteration in the proportion of cells which were positive for any subset marker or in the ratio of helper/inducer to cytotoxic/suppressor T cells compared to controls. Measles patients, however, had a greater number of cells expressing the activation antigens T10 and IL-2 receptor than controls. Incorporation of [3H]thymidine was also higher in measles patients when exogenous natural or recombinant IL-2 was added to unstimulated cultured cells. We conclude that the peripheral blood mononuclear leukocytes of patients with measles have normal proportions of helper/inducer and cytotoxic/suppressor T lymphocytes, B lymphocytes, and monocytes but that an increased number of these cells are in an activated state.

UR - http://www.scopus.com/inward/record.url?scp=0022534121&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022534121&partnerID=8YFLogxK

U2 - 10.1016/0090-1229(86)90035-8

DO - 10.1016/0090-1229(86)90035-8

M3 - Article

C2 - 3087668

AN - SCOPUS:0022534121

VL - 40

SP - 305

EP - 312

JO - Clinical Immunology

JF - Clinical Immunology

SN - 1521-6616

IS - 2

ER -