Peripheral blood mononuclear cell collection from patients undergoing adoptive immunotherapy or peripheral blood-derived stem cell transplantation and from healthy donors

U. Keilholz, H. G. Klein, M. Korbling, B. Brado, C. S. Carter, H. Cullis, F. Galm, W. Hunstein

Research output: Contribution to journalArticlepeer-review

Abstract

The demand for collection of mononuclear cells from the peripheral blood of patients for therapeutic purpose is rapidly increasing. Automated blood cell separators are usually designed for colletion of blood components from healthy donors. We reviewed safety and efficiency of collection data of a new procedure for the Fenwal CS 3000 blood cell separator in 125 collections from normal donors and 101 collections from patients after IL-2 pretreatment or chemotherapy. The new procedure set red blood cell spillovers to occur at 3.5 minture intervals, using procedure 1 with the interface detector set at 1,000 and the standard granulocyte and collection chambers. Despite significant anemia and thrombocytopenia in a large number of patients no serious procedure-related side effects occurred. The lymphocyte yield was 4.74 ± 1.6 x 109 per 5 liters of blood processed in normal donors and 24.2 ± 12.0 x 109 per 10 liters of blood processed after Il-2 treatment. After chemotherapy the lymphocyte yield was 4.5 ± 3.1 x 109 per 10 liters of blood processed; the collection efficiency was found to be significantly lower in this group. The main problem was teh platelet loss of 35.6 ± 12% of the initial count in normal donors, 40.3 ± 14.1% after Il-2 treatment, and 42.1 ± 18.0% after chemotherapy. The platelet loss is, however, closely related to the preapheresis platelet count; patients with thrmbocytopenia lose fewer platelets than normal donors. Therefore the procedure was found to be safe for patients with a platelet count as low as 20/nl. The report providessd a basis for safe, effective mononuclear cell collection from patients with very abnormal peripheral blood counts.

Original languageEnglish (US)
Pages (from-to)131-136
Number of pages6
JournalJournal of Clinical Apheresis
Volume6
Issue number3
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Hematology

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