TY - JOUR
T1 - Peripheral and central pathways regulating the kidney
T2 - a study using pseudorabies virus
AU - Schramm, Lawrence P.
AU - Strack, Alison M.
AU - Platt, Kenneth B.
AU - Loewy, Arthur D.
N1 - Funding Information:
Acknowledgements. We thank William Sawyer, John O'Reilly, Helen Wilfehrt and Hanna Rodriguez for their expert technical assistance. Part of this research was conducted in St. Louis, MO by L.P.S. with funding provided by a Tilghman Travelling Fellowship from the Johns Hopkins School of Medicine. This research was supported by NIH grants HL16315 (to L.P.S.) and HL25449( to A.D.L) and by the Iowa Livestock Advisory Committee (to K.B.P.).
PY - 1993/7/9
Y1 - 1993/7/9
N2 - We used the retrograde transneuronal transport of a neurotropic virus, pseudorabies virus (PRV), to identify the neurons in sympathetic ganglia, spinal cord and brain which regulate renal function and renal circulation. PRV was microinjected into the left kidney of 70, pentobarbital-anesthetized, male rats. After an incubation period of 1-4 days, rats were anesthetized and sacrificed. PRV-infected neurons were located immunocytochemically in pre- and paravertebral sympathetic ganglia, the intermediolateral cell column of the T10-T13 segments and several brainstem cell groups: the medullary raphe nuclei, rostral ventrolateral medulla, rostral ventromedial medulla, A5 cell group, and the paraventricular hypothalamic nucleus. In more heavily infected rats, additional labeling was found in the locus coeruleus, periadqueductal gray matter, lateral hypothalamic area, zona incerta, and anterior hypothalamic area. No infected propriospinal neurons were observed in the lateral spinal nucleus or gray matter of the caudal cervical, lumbosacral or thoracic spinal segments not containing infected putative sympathetic preganglionic neurons. The paucity of infected propiospinal neurons in the presence of infected brainstem neurons, even in lightly infected rats, is discussed in reference to the relative importance of descending vs spinal regulation of the sympathetic outflow to the kidney.
AB - We used the retrograde transneuronal transport of a neurotropic virus, pseudorabies virus (PRV), to identify the neurons in sympathetic ganglia, spinal cord and brain which regulate renal function and renal circulation. PRV was microinjected into the left kidney of 70, pentobarbital-anesthetized, male rats. After an incubation period of 1-4 days, rats were anesthetized and sacrificed. PRV-infected neurons were located immunocytochemically in pre- and paravertebral sympathetic ganglia, the intermediolateral cell column of the T10-T13 segments and several brainstem cell groups: the medullary raphe nuclei, rostral ventrolateral medulla, rostral ventromedial medulla, A5 cell group, and the paraventricular hypothalamic nucleus. In more heavily infected rats, additional labeling was found in the locus coeruleus, periadqueductal gray matter, lateral hypothalamic area, zona incerta, and anterior hypothalamic area. No infected propriospinal neurons were observed in the lateral spinal nucleus or gray matter of the caudal cervical, lumbosacral or thoracic spinal segments not containing infected putative sympathetic preganglionic neurons. The paucity of infected propiospinal neurons in the presence of infected brainstem neurons, even in lightly infected rats, is discussed in reference to the relative importance of descending vs spinal regulation of the sympathetic outflow to the kidney.
KW - Intermediolateral cell column
KW - Kidney
KW - Postganglionic neuron
KW - Renal nerve
KW - Retrograde tracing
KW - Sympathetic preganglionic neuron
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U2 - 10.1016/0006-8993(93)90216-A
DO - 10.1016/0006-8993(93)90216-A
M3 - Article
C2 - 7689411
AN - SCOPUS:0027207765
VL - 616
SP - 251
EP - 262
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -