Periodic DNA patrolling underlies diverse functions of Pif1 on R-loops and G-rich DNA

Ruobo Zhou, Jichuan Zhang, Matthew L. Bochman, Virginia A. Zakian, Taekjip Ha

Research output: Contribution to journalArticle

Abstract

Pif1 family helicases are conserved from bacteria to humans. Here, we report a novel DNA patrolling activity which may underlie Pif1's diverse functions: a Pif1 monomer preferentially anchors itself to a 3'-tailed DNA junction and periodically reel in the 3' tail with a step size of one nucleotide, extruding a loop. This periodic patrolling activity is used to unfold an intramolecular G-quadruplex (G4) structure on every encounter, and is sufficient to unwind RNA-DNA heteroduplex but not duplex DNA. Instead of leaving after G4 unwinding, allowing it to refold, or going beyond to unwind duplex DNA, Pif1 repeatedly unwinds G4 DNA, keeping it unfolded. Pif1-induced unfolding of G4 occurs in three discrete steps, one strand at a time, and is powerful enough to overcome G4-stabilizing drugs. The periodic patrolling activity may keep Pif1 at its site of in vivo action in displacing telomerase, resolving R-loops, and keeping G4 unfolded during replication, recombination and repair.

Original languageEnglish (US)
Article numbere02190
JournaleLife
Volume2014
Issue number3
DOIs
StatePublished - Apr 29 2014
Externally publishedYes

Fingerprint

DNA
Nucleic Acid Heteroduplexes
G-Quadruplexes
Recombinational DNA Repair
Telomerase
Anchors
Tail
Bacteria
Repair
Nucleotides
Monomers
RNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Periodic DNA patrolling underlies diverse functions of Pif1 on R-loops and G-rich DNA. / Zhou, Ruobo; Zhang, Jichuan; Bochman, Matthew L.; Zakian, Virginia A.; Ha, Taekjip.

In: eLife, Vol. 2014, No. 3, e02190, 29.04.2014.

Research output: Contribution to journalArticle

Zhou, Ruobo ; Zhang, Jichuan ; Bochman, Matthew L. ; Zakian, Virginia A. ; Ha, Taekjip. / Periodic DNA patrolling underlies diverse functions of Pif1 on R-loops and G-rich DNA. In: eLife. 2014 ; Vol. 2014, No. 3.
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