Perikaryal RNA changes within neurons of the caudate-putamen and substantia nigra in soman intoxicated rats

L. J. Martin; J. A. Doebler; A. Anthony

doi:10.1016/0197-0186(86)90040-9

Perikaryal RNA changes within neurons of the caudate-putamen and substantia nigra in soman intoxicated rats

L. J. Martin, J. A. Doebler, A. Anthony

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Quantitative azure B cytophotometry was employed to monitor neuronal ribonucleic acid (RNA) metabolism within cholinergic and noncholinergic brain compartments following single sc injections of either 0.5, 0.9 or 1.5 LD₅₀ soman (pinacolyl methylphosphonofluoridate). Dose-dependent losses in neuronal RNA were observed within the cholinergic caudate-putamen (CP) and dopaminergic substantia nigra pars compacta (SNPC), whereas levels of RNA were generally maintained or elevated within the gabaergic substantia nigra pars reticulata (SNPR). CP acetylcholinesterase was inhibited in a dose-dependent fashion. These neuronal RNA changes are perhaps related to seizure activity. The overall data lend support to the hypothesis that alterations in noncholinergic activity contribute to certain manifestations of soman neurotoxicity, such as seizures, which probably stem from an impairment in the functional integrity of both excitatory and inhibitory neuronal elements.

Original language	English (US)
Pages (from-to)	121-126
Number of pages	6
Journal	Neurochemistry International
Volume	9
Issue number	1
DOIs	https://doi.org/10.1016/0197-0186(86)90040-9
State	Published - 1986
Externally published	Yes

ASJC Scopus subject areas

Cellular and Molecular Neuroscience
Cell Biology

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10.1016/0197-0186(86)90040-9

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title = "Perikaryal RNA changes within neurons of the caudate-putamen and substantia nigra in soman intoxicated rats",

abstract = "Quantitative azure B cytophotometry was employed to monitor neuronal ribonucleic acid (RNA) metabolism within cholinergic and noncholinergic brain compartments following single sc injections of either 0.5, 0.9 or 1.5 LD50 soman (pinacolyl methylphosphonofluoridate). Dose-dependent losses in neuronal RNA were observed within the cholinergic caudate-putamen (CP) and dopaminergic substantia nigra pars compacta (SNPC), whereas levels of RNA were generally maintained or elevated within the gabaergic substantia nigra pars reticulata (SNPR). CP acetylcholinesterase was inhibited in a dose-dependent fashion. These neuronal RNA changes are perhaps related to seizure activity. The overall data lend support to the hypothesis that alterations in noncholinergic activity contribute to certain manifestations of soman neurotoxicity, such as seizures, which probably stem from an impairment in the functional integrity of both excitatory and inhibitory neuronal elements.",

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T1 - Perikaryal RNA changes within neurons of the caudate-putamen and substantia nigra in soman intoxicated rats

AU - Martin, L. J.

AU - Doebler, J. A.

AU - Anthony, A.

PY - 1986

Y1 - 1986

N2 - Quantitative azure B cytophotometry was employed to monitor neuronal ribonucleic acid (RNA) metabolism within cholinergic and noncholinergic brain compartments following single sc injections of either 0.5, 0.9 or 1.5 LD50 soman (pinacolyl methylphosphonofluoridate). Dose-dependent losses in neuronal RNA were observed within the cholinergic caudate-putamen (CP) and dopaminergic substantia nigra pars compacta (SNPC), whereas levels of RNA were generally maintained or elevated within the gabaergic substantia nigra pars reticulata (SNPR). CP acetylcholinesterase was inhibited in a dose-dependent fashion. These neuronal RNA changes are perhaps related to seizure activity. The overall data lend support to the hypothesis that alterations in noncholinergic activity contribute to certain manifestations of soman neurotoxicity, such as seizures, which probably stem from an impairment in the functional integrity of both excitatory and inhibitory neuronal elements.

AB - Quantitative azure B cytophotometry was employed to monitor neuronal ribonucleic acid (RNA) metabolism within cholinergic and noncholinergic brain compartments following single sc injections of either 0.5, 0.9 or 1.5 LD50 soman (pinacolyl methylphosphonofluoridate). Dose-dependent losses in neuronal RNA were observed within the cholinergic caudate-putamen (CP) and dopaminergic substantia nigra pars compacta (SNPC), whereas levels of RNA were generally maintained or elevated within the gabaergic substantia nigra pars reticulata (SNPR). CP acetylcholinesterase was inhibited in a dose-dependent fashion. These neuronal RNA changes are perhaps related to seizure activity. The overall data lend support to the hypothesis that alterations in noncholinergic activity contribute to certain manifestations of soman neurotoxicity, such as seizures, which probably stem from an impairment in the functional integrity of both excitatory and inhibitory neuronal elements.

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Perikaryal RNA changes within neurons of the caudate-putamen and substantia nigra in soman intoxicated rats

Abstract

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