Pericytic-like angiotropism of glioma and melanoma cells

Claire Lugassy, Raymond I. Haroun, Henry Brem, Betty M. Tyler, Robert V. Jones, Patricia M. Fernandez, Steven R. Patierno, Hynda K. Kleinman, Raymond L. Barnhill

Research output: Contribution to journalArticle

Abstract

We have identified in malignant melanoma an angiotumoral complex in which tumor cells occupy a pericytic location along the endothelium of microvessels without evidence of intravasation. We have suggested that this pericytic-like angiotropism could be a marker of an extravascular migration of tumor cells along the abluminal surface of vessels. The extravascular migratory metastasis proposed for melanoma has close analogies with glioma migration. To compare our hypothesis of extravascular migration by melanoma with the migration of glioma cells, we have used the B16 murine melanoma cell line and the GL26 murine glioma cell line in an in vivo murine brain tumor model and in vitro using endothelial cells that have formed capillary-like structures and have been cocultivated with tumor cells. In the brain tumors, a clear progression of glioma and melanoma cells was observed along the abluminal surface of vessels, where they occupied a pericytic location along the periendothelial laminin. In vitro, time-lapse videomicroscopy recorded the migration of tumor cells toward endothelial tubules. After 24 hours, both the melanoma cells and the glioma cells were localized along the external surfaces of the vascular tubules, occupying a pericytic-like location. These similarities between glioma and melanoma support the hypothesis of an extravascular migration of melanoma cells, particularly along the abluminal surface of vessels.

Original languageEnglish (US)
Pages (from-to)473-478
Number of pages6
JournalAmerican Journal of Dermatopathology
Volume24
Issue number6
DOIs
StatePublished - Dec 1 2002

Keywords

  • Angiotumoral complex
  • Extravascular migratory metastasis
  • Glioma
  • Laminin
  • Melanoma
  • Pericytic-like angiotropism

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Dermatology

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  • Cite this

    Lugassy, C., Haroun, R. I., Brem, H., Tyler, B. M., Jones, R. V., Fernandez, P. M., Patierno, S. R., Kleinman, H. K., & Barnhill, R. L. (2002). Pericytic-like angiotropism of glioma and melanoma cells. American Journal of Dermatopathology, 24(6), 473-478. https://doi.org/10.1097/00000372-200212000-00003