TY - JOUR
T1 - Pericytic-like angiotropism of glioma and melanoma cells
AU - Lugassy, Claire
AU - Haroun, Raymond I.
AU - Brem, Henry
AU - Tyler, Betty M.
AU - Jones, Robert V.
AU - Fernandez, Patricia M.
AU - Patierno, Steven R.
AU - Kleinman, Hynda K.
AU - Barnhill, Raymond L.
PY - 2002/12
Y1 - 2002/12
N2 - We have identified in malignant melanoma an angiotumoral complex in which tumor cells occupy a pericytic location along the endothelium of microvessels without evidence of intravasation. We have suggested that this pericytic-like angiotropism could be a marker of an extravascular migration of tumor cells along the abluminal surface of vessels. The extravascular migratory metastasis proposed for melanoma has close analogies with glioma migration. To compare our hypothesis of extravascular migration by melanoma with the migration of glioma cells, we have used the B16 murine melanoma cell line and the GL26 murine glioma cell line in an in vivo murine brain tumor model and in vitro using endothelial cells that have formed capillary-like structures and have been cocultivated with tumor cells. In the brain tumors, a clear progression of glioma and melanoma cells was observed along the abluminal surface of vessels, where they occupied a pericytic location along the periendothelial laminin. In vitro, time-lapse videomicroscopy recorded the migration of tumor cells toward endothelial tubules. After 24 hours, both the melanoma cells and the glioma cells were localized along the external surfaces of the vascular tubules, occupying a pericytic-like location. These similarities between glioma and melanoma support the hypothesis of an extravascular migration of melanoma cells, particularly along the abluminal surface of vessels.
AB - We have identified in malignant melanoma an angiotumoral complex in which tumor cells occupy a pericytic location along the endothelium of microvessels without evidence of intravasation. We have suggested that this pericytic-like angiotropism could be a marker of an extravascular migration of tumor cells along the abluminal surface of vessels. The extravascular migratory metastasis proposed for melanoma has close analogies with glioma migration. To compare our hypothesis of extravascular migration by melanoma with the migration of glioma cells, we have used the B16 murine melanoma cell line and the GL26 murine glioma cell line in an in vivo murine brain tumor model and in vitro using endothelial cells that have formed capillary-like structures and have been cocultivated with tumor cells. In the brain tumors, a clear progression of glioma and melanoma cells was observed along the abluminal surface of vessels, where they occupied a pericytic location along the periendothelial laminin. In vitro, time-lapse videomicroscopy recorded the migration of tumor cells toward endothelial tubules. After 24 hours, both the melanoma cells and the glioma cells were localized along the external surfaces of the vascular tubules, occupying a pericytic-like location. These similarities between glioma and melanoma support the hypothesis of an extravascular migration of melanoma cells, particularly along the abluminal surface of vessels.
KW - Angiotumoral complex
KW - Extravascular migratory metastasis
KW - Glioma
KW - Laminin
KW - Melanoma
KW - Pericytic-like angiotropism
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U2 - 10.1097/00000372-200212000-00003
DO - 10.1097/00000372-200212000-00003
M3 - Article
C2 - 12454598
AN - SCOPUS:0036895210
SN - 0193-1091
VL - 24
SP - 473
EP - 478
JO - American Journal of Dermatopathology
JF - American Journal of Dermatopathology
IS - 6
ER -