TY - JOUR
T1 - Perfusion defect size predicts engraftment but not early retention of intra-myocardially injected cardiosphere-derived cells after acute myocardial infarction
AU - Lautamäki, Riikka
AU - Terrovitis, John
AU - Bonios, Michael
AU - Yu, Jianhua
AU - Tsui, Benjamin M.
AU - Roselle Abraham, M.
AU - Bengel, Frank M.
N1 - Funding Information:
This study was supported by NIH grant 1RO1HL092985-01A2 (FMB and MRA). Dr. Lautamäki was supported by grants from The Finnish Cardiac Research Foundation, The Finnish Medical Foundation, The Instrumentarium Foundation for Science, and by a Bracco/SNM Research Fellowship in Cardiovascular Molecular Imaging.
PY - 2011/11
Y1 - 2011/11
N2 - Therapeutic cell retention and engraftment are critical for myocardial regeneration. Underlying mechanisms, including the role of tissue perfusion, are not well understood. In Wistar Kyoto rats, syngeneic cardiospherederived cells (CDCs) were injected intramyocardially, after experimental myocardial infarction. CDCs were labeled with [ 18F]-FDG (n = 7), for quantification of 1-h retention, or with sodium-iodide-symporter gene (NIS; n = 8), for detection of 24-h engraftment by reporter imaging. Perfusion was imaged simultaneously. Infarct size was 37 ± 9 and 38 ± 9% of LV in FDG and NIS groups. Cell signal was located in the infarct border zone in all animals. No significant relationship was observed between infarct size and 1-h CDC retention (r = -0.65; P = 0.11). However, infarct size correlated significantly with 24-h engraftment (r = 0.75; P = 0.03). Residual perfusion at the injection site was not related to cell retention/engraftment. Larger infarcts are associated with improved CDC engraftment. This observation encourages further investigation of microenvironmental conditions after ischemic damage and their role in therapeutic cell survival.
AB - Therapeutic cell retention and engraftment are critical for myocardial regeneration. Underlying mechanisms, including the role of tissue perfusion, are not well understood. In Wistar Kyoto rats, syngeneic cardiospherederived cells (CDCs) were injected intramyocardially, after experimental myocardial infarction. CDCs were labeled with [ 18F]-FDG (n = 7), for quantification of 1-h retention, or with sodium-iodide-symporter gene (NIS; n = 8), for detection of 24-h engraftment by reporter imaging. Perfusion was imaged simultaneously. Infarct size was 37 ± 9 and 38 ± 9% of LV in FDG and NIS groups. Cell signal was located in the infarct border zone in all animals. No significant relationship was observed between infarct size and 1-h CDC retention (r = -0.65; P = 0.11). However, infarct size correlated significantly with 24-h engraftment (r = 0.75; P = 0.03). Residual perfusion at the injection site was not related to cell retention/engraftment. Larger infarcts are associated with improved CDC engraftment. This observation encourages further investigation of microenvironmental conditions after ischemic damage and their role in therapeutic cell survival.
KW - Cardiosphere-derived cells
KW - Cell tracking
KW - Myocardial perfusion
KW - Myocardial regeneration
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U2 - 10.1007/s00395-011-0197-5
DO - 10.1007/s00395-011-0197-5
M3 - Article
C2 - 21706191
AN - SCOPUS:84856711033
SN - 0300-8428
VL - 106
SP - 1379
EP - 1386
JO - Basic Research in Cardiology
JF - Basic Research in Cardiology
IS - 6
ER -