Performance of the MagNA Pure LC robot for extraction of Chlamydia trachomatis and Neisseria gonorrhoeae DNA from urine and swab specimens

Nicholas Dalesio, Vince Marsiglia, Andrew Quinn, Thomas C Quinn, Charlotte A Gaydos

Research output: Contribution to journalArticle

Abstract

DNA from uncentrifuged urines (n = 195 and n = 202) and cervical swabs (n = 221 and n = 601) was extracted by the MagNA Pure LC robot and the Amplicor method to validate the robot's extraction for Chlamydia trachomatis and Neisseria gonorrhoeae testing by Roche PCR. The robot provided a highly sensitive and specific method of DNA extraction.

Original languageEnglish (US)
Pages (from-to)3300-3302
Number of pages3
JournalJournal of Clinical Microbiology
Volume42
Issue number7
DOIs
StatePublished - Jul 2004

Fingerprint

Neisseria gonorrhoeae
Chlamydia trachomatis
Urine
DNA
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

@article{307dd50200c940829a0543e8bcc25070,
title = "Performance of the MagNA Pure LC robot for extraction of Chlamydia trachomatis and Neisseria gonorrhoeae DNA from urine and swab specimens",
abstract = "DNA from uncentrifuged urines (n = 195 and n = 202) and cervical swabs (n = 221 and n = 601) was extracted by the MagNA Pure LC robot and the Amplicor method to validate the robot's extraction for Chlamydia trachomatis and Neisseria gonorrhoeae testing by Roche PCR. The robot provided a highly sensitive and specific method of DNA extraction.",
author = "Nicholas Dalesio and Vince Marsiglia and Andrew Quinn and Quinn, {Thomas C} and Gaydos, {Charlotte A}",
year = "2004",
month = "7",
doi = "10.1128/JCM.42.7.3300-3302.2004",
language = "English (US)",
volume = "42",
pages = "3300--3302",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "7",

}

TY - JOUR

T1 - Performance of the MagNA Pure LC robot for extraction of Chlamydia trachomatis and Neisseria gonorrhoeae DNA from urine and swab specimens

AU - Dalesio, Nicholas

AU - Marsiglia, Vince

AU - Quinn, Andrew

AU - Quinn, Thomas C

AU - Gaydos, Charlotte A

PY - 2004/7

Y1 - 2004/7

N2 - DNA from uncentrifuged urines (n = 195 and n = 202) and cervical swabs (n = 221 and n = 601) was extracted by the MagNA Pure LC robot and the Amplicor method to validate the robot's extraction for Chlamydia trachomatis and Neisseria gonorrhoeae testing by Roche PCR. The robot provided a highly sensitive and specific method of DNA extraction.

AB - DNA from uncentrifuged urines (n = 195 and n = 202) and cervical swabs (n = 221 and n = 601) was extracted by the MagNA Pure LC robot and the Amplicor method to validate the robot's extraction for Chlamydia trachomatis and Neisseria gonorrhoeae testing by Roche PCR. The robot provided a highly sensitive and specific method of DNA extraction.

UR - http://www.scopus.com/inward/record.url?scp=3142777739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3142777739&partnerID=8YFLogxK

U2 - 10.1128/JCM.42.7.3300-3302.2004

DO - 10.1128/JCM.42.7.3300-3302.2004

M3 - Article

VL - 42

SP - 3300

EP - 3302

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 7

ER -