Performance of the Euroline Western blot assay in the detection of herpes simplex virus type 2 antibody in Uganda, China and the USA

J. D. Neal, Aaron A Tobian, Oliver B. Laeyendecker, T. D. Ngo, Andrew Redd, Steven James Reynolds, R. Ashley Morrow, J. L. Manucci, D. Serwadda, Ronald H Gray, Thomas C Quinn

Research output: Contribution to journalArticle

Abstract

Herpes simplex virus type 2 (HSV-2) infection is one of the most common sexually transmitted infections (STIs) worldwide. While glycoprotein G-2 enzyme-linked immunosorbent assays are commonly used for the serological detection of HSV-2 antibodies, they have low specificity in developing countries. The Euroline Western blot (WB) is a commercially available assay that is easy to perform; however, little is known about its performance characteristics. This study evaluated Euroline WB for the detection of HSV-2 antibodies compared with University of Washington Western blot in three geographically different regions: Baltimore, MD, USA; Rakai, Uganda; and Kunming, China. Among the 135 American men attending a STI clinic in Baltimore, MD, 72% (n = 97) were HSV-2-positive by Euroline WB, showing a sensitivity of 97.8% and a specificity of 81.8%. Among the 273 commercial sex workers in Kunming, 62.3% were HSV-2-positive by Euroline WB (sensitivity 96.9%, specificity 89.1%). Among the 437 Ugandans in Rakai, 67.3% were HSV-2-positive by Euroline WB (sensitivity 98.7%, specificity 65.4%). The Euroline WB has a consistently high sensitivity, but specificity varied significantly among the different locations.

Original languageEnglish (US)
Pages (from-to)342-344
Number of pages3
JournalInternational Journal of STD and AIDS
Volume22
Issue number6
DOIs
StatePublished - Jun 2011

Fingerprint

Human Herpesvirus 2
Uganda
China
Western Blotting
Antibodies
Baltimore
Sexually Transmitted Diseases
Sensitivity and Specificity
Sex Workers
Virus Diseases
Developing Countries
Glycoproteins
Enzyme-Linked Immunosorbent Assay

Keywords

  • Antibody
  • Diagnosis
  • Enzyme-linked immunosorbent assays (ELISAs)
  • Euroline
  • Genital herpes
  • Herpes simplex virus type 2 (HSV-2)
  • Serologic diagnostic assays
  • Western blot

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

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title = "Performance of the Euroline Western blot assay in the detection of herpes simplex virus type 2 antibody in Uganda, China and the USA",
abstract = "Herpes simplex virus type 2 (HSV-2) infection is one of the most common sexually transmitted infections (STIs) worldwide. While glycoprotein G-2 enzyme-linked immunosorbent assays are commonly used for the serological detection of HSV-2 antibodies, they have low specificity in developing countries. The Euroline Western blot (WB) is a commercially available assay that is easy to perform; however, little is known about its performance characteristics. This study evaluated Euroline WB for the detection of HSV-2 antibodies compared with University of Washington Western blot in three geographically different regions: Baltimore, MD, USA; Rakai, Uganda; and Kunming, China. Among the 135 American men attending a STI clinic in Baltimore, MD, 72{\%} (n = 97) were HSV-2-positive by Euroline WB, showing a sensitivity of 97.8{\%} and a specificity of 81.8{\%}. Among the 273 commercial sex workers in Kunming, 62.3{\%} were HSV-2-positive by Euroline WB (sensitivity 96.9{\%}, specificity 89.1{\%}). Among the 437 Ugandans in Rakai, 67.3{\%} were HSV-2-positive by Euroline WB (sensitivity 98.7{\%}, specificity 65.4{\%}). The Euroline WB has a consistently high sensitivity, but specificity varied significantly among the different locations.",
keywords = "Antibody, Diagnosis, Enzyme-linked immunosorbent assays (ELISAs), Euroline, Genital herpes, Herpes simplex virus type 2 (HSV-2), Serologic diagnostic assays, Western blot",
author = "Neal, {J. D.} and Tobian, {Aaron A} and Laeyendecker, {Oliver B.} and Ngo, {T. D.} and Andrew Redd and Reynolds, {Steven James} and Morrow, {R. Ashley} and Manucci, {J. L.} and D. Serwadda and Gray, {Ronald H} and Quinn, {Thomas C}",
year = "2011",
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T1 - Performance of the Euroline Western blot assay in the detection of herpes simplex virus type 2 antibody in Uganda, China and the USA

AU - Neal, J. D.

AU - Tobian, Aaron A

AU - Laeyendecker, Oliver B.

AU - Ngo, T. D.

AU - Redd, Andrew

AU - Reynolds, Steven James

AU - Morrow, R. Ashley

AU - Manucci, J. L.

AU - Serwadda, D.

AU - Gray, Ronald H

AU - Quinn, Thomas C

PY - 2011/6

Y1 - 2011/6

N2 - Herpes simplex virus type 2 (HSV-2) infection is one of the most common sexually transmitted infections (STIs) worldwide. While glycoprotein G-2 enzyme-linked immunosorbent assays are commonly used for the serological detection of HSV-2 antibodies, they have low specificity in developing countries. The Euroline Western blot (WB) is a commercially available assay that is easy to perform; however, little is known about its performance characteristics. This study evaluated Euroline WB for the detection of HSV-2 antibodies compared with University of Washington Western blot in three geographically different regions: Baltimore, MD, USA; Rakai, Uganda; and Kunming, China. Among the 135 American men attending a STI clinic in Baltimore, MD, 72% (n = 97) were HSV-2-positive by Euroline WB, showing a sensitivity of 97.8% and a specificity of 81.8%. Among the 273 commercial sex workers in Kunming, 62.3% were HSV-2-positive by Euroline WB (sensitivity 96.9%, specificity 89.1%). Among the 437 Ugandans in Rakai, 67.3% were HSV-2-positive by Euroline WB (sensitivity 98.7%, specificity 65.4%). The Euroline WB has a consistently high sensitivity, but specificity varied significantly among the different locations.

AB - Herpes simplex virus type 2 (HSV-2) infection is one of the most common sexually transmitted infections (STIs) worldwide. While glycoprotein G-2 enzyme-linked immunosorbent assays are commonly used for the serological detection of HSV-2 antibodies, they have low specificity in developing countries. The Euroline Western blot (WB) is a commercially available assay that is easy to perform; however, little is known about its performance characteristics. This study evaluated Euroline WB for the detection of HSV-2 antibodies compared with University of Washington Western blot in three geographically different regions: Baltimore, MD, USA; Rakai, Uganda; and Kunming, China. Among the 135 American men attending a STI clinic in Baltimore, MD, 72% (n = 97) were HSV-2-positive by Euroline WB, showing a sensitivity of 97.8% and a specificity of 81.8%. Among the 273 commercial sex workers in Kunming, 62.3% were HSV-2-positive by Euroline WB (sensitivity 96.9%, specificity 89.1%). Among the 437 Ugandans in Rakai, 67.3% were HSV-2-positive by Euroline WB (sensitivity 98.7%, specificity 65.4%). The Euroline WB has a consistently high sensitivity, but specificity varied significantly among the different locations.

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