Performance of the BioPlex 2200 HIV Ag-Ab assay for identifying acute HIV infection

Susan Eshleman, Estelle Piwowar-Manning, Mariya V. Sivay, Barbara Debevec, Stephanie Veater, Laura McKinstry, Linda Gail Bekker, Sharon Mannheimer, Robert M. Grant, Margaret A. Chesney, Thomas J. Coates, Beryl A. Koblin, Jessica Marie Louise Fogel

Research output: Contribution to journalArticle

Abstract

Background Assays that detect HIV antigen (Ag) and antibody (Ab) can be used to screen for HIV infection. Objectives To compare the performance of the BioPlex 2200 HIV Ag-Ab assay and two other Ag/Ab combination assays for detection of acute HIV infection. Study design Samples were obtained from 24 individuals (18 from the US, 6 from South Africa); these individuals were classified as having acute infection based on the following criteria: positive qualitative RNA assay; two negative rapid tests; negative discriminatory test. The samples were tested with the BioPlex assay, the ARCHITECT HIV Ag/Ab Combo test, the Bio-Rad GS HIV Combo Ag-Ab EIA test, and a viral load assay. Results Twelve (50.0%) of 24 samples had RNA detected only (> 40 to 13,476 copies/mL). Ten (43.5%) samples had reactive results with all three Ag/Ab assays, one sample was reactive with the ARCHITECT and Bio-Rad assays, and one sample was reactive with the Bio-Rad and BioPlex assays. The 11 samples that were reactive with the BioPlex assay had viral loads from 83,010 to >750,000 copies/mL; 9/11 samples were classified as Ag positive/Ab negative by the BioPlex assay. Conclusions Detection of acute HIV infection was similar for the BioPlex assay and two other Ag/Ab assays. All three tests were less sensitive than a qualitative RNA assay and only detected HIV Ag when the viral load was high. The BioPlex assay detected acute infection in about half of the cases, and identified most of those infections as Ag positive/Ab negative.

Original languageEnglish (US)
Pages (from-to)67-70
Number of pages4
JournalJournal of Clinical Virology
Volume99-100
DOIs
StatePublished - Feb 1 2018

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HIV Antigens
HIV Antibodies
HIV Infections
Antibodies
Antigens
Viral Load
RNA
Infection
South Africa
bioplex

Keywords

  • Acute
  • Ag/Ab assay
  • BioPlex
  • HIV

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Performance of the BioPlex 2200 HIV Ag-Ab assay for identifying acute HIV infection. / Eshleman, Susan; Piwowar-Manning, Estelle; Sivay, Mariya V.; Debevec, Barbara; Veater, Stephanie; McKinstry, Laura; Bekker, Linda Gail; Mannheimer, Sharon; Grant, Robert M.; Chesney, Margaret A.; Coates, Thomas J.; Koblin, Beryl A.; Fogel, Jessica Marie Louise.

In: Journal of Clinical Virology, Vol. 99-100, 01.02.2018, p. 67-70.

Research output: Contribution to journalArticle

Eshleman, S, Piwowar-Manning, E, Sivay, MV, Debevec, B, Veater, S, McKinstry, L, Bekker, LG, Mannheimer, S, Grant, RM, Chesney, MA, Coates, TJ, Koblin, BA & Fogel, JML 2018, 'Performance of the BioPlex 2200 HIV Ag-Ab assay for identifying acute HIV infection', Journal of Clinical Virology, vol. 99-100, pp. 67-70. https://doi.org/10.1016/j.jcv.2018.01.003
Eshleman, Susan ; Piwowar-Manning, Estelle ; Sivay, Mariya V. ; Debevec, Barbara ; Veater, Stephanie ; McKinstry, Laura ; Bekker, Linda Gail ; Mannheimer, Sharon ; Grant, Robert M. ; Chesney, Margaret A. ; Coates, Thomas J. ; Koblin, Beryl A. ; Fogel, Jessica Marie Louise. / Performance of the BioPlex 2200 HIV Ag-Ab assay for identifying acute HIV infection. In: Journal of Clinical Virology. 2018 ; Vol. 99-100. pp. 67-70.
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abstract = "Background Assays that detect HIV antigen (Ag) and antibody (Ab) can be used to screen for HIV infection. Objectives To compare the performance of the BioPlex 2200 HIV Ag-Ab assay and two other Ag/Ab combination assays for detection of acute HIV infection. Study design Samples were obtained from 24 individuals (18 from the US, 6 from South Africa); these individuals were classified as having acute infection based on the following criteria: positive qualitative RNA assay; two negative rapid tests; negative discriminatory test. The samples were tested with the BioPlex assay, the ARCHITECT HIV Ag/Ab Combo test, the Bio-Rad GS HIV Combo Ag-Ab EIA test, and a viral load assay. Results Twelve (50.0{\%}) of 24 samples had RNA detected only (> 40 to 13,476 copies/mL). Ten (43.5{\%}) samples had reactive results with all three Ag/Ab assays, one sample was reactive with the ARCHITECT and Bio-Rad assays, and one sample was reactive with the Bio-Rad and BioPlex assays. The 11 samples that were reactive with the BioPlex assay had viral loads from 83,010 to >750,000 copies/mL; 9/11 samples were classified as Ag positive/Ab negative by the BioPlex assay. Conclusions Detection of acute HIV infection was similar for the BioPlex assay and two other Ag/Ab assays. All three tests were less sensitive than a qualitative RNA assay and only detected HIV Ag when the viral load was high. The BioPlex assay detected acute infection in about half of the cases, and identified most of those infections as Ag positive/Ab negative.",
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AU - Eshleman, Susan

AU - Piwowar-Manning, Estelle

AU - Sivay, Mariya V.

AU - Debevec, Barbara

AU - Veater, Stephanie

AU - McKinstry, Laura

AU - Bekker, Linda Gail

AU - Mannheimer, Sharon

AU - Grant, Robert M.

AU - Chesney, Margaret A.

AU - Coates, Thomas J.

AU - Koblin, Beryl A.

AU - Fogel, Jessica Marie Louise

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N2 - Background Assays that detect HIV antigen (Ag) and antibody (Ab) can be used to screen for HIV infection. Objectives To compare the performance of the BioPlex 2200 HIV Ag-Ab assay and two other Ag/Ab combination assays for detection of acute HIV infection. Study design Samples were obtained from 24 individuals (18 from the US, 6 from South Africa); these individuals were classified as having acute infection based on the following criteria: positive qualitative RNA assay; two negative rapid tests; negative discriminatory test. The samples were tested with the BioPlex assay, the ARCHITECT HIV Ag/Ab Combo test, the Bio-Rad GS HIV Combo Ag-Ab EIA test, and a viral load assay. Results Twelve (50.0%) of 24 samples had RNA detected only (> 40 to 13,476 copies/mL). Ten (43.5%) samples had reactive results with all three Ag/Ab assays, one sample was reactive with the ARCHITECT and Bio-Rad assays, and one sample was reactive with the Bio-Rad and BioPlex assays. The 11 samples that were reactive with the BioPlex assay had viral loads from 83,010 to >750,000 copies/mL; 9/11 samples were classified as Ag positive/Ab negative by the BioPlex assay. Conclusions Detection of acute HIV infection was similar for the BioPlex assay and two other Ag/Ab assays. All three tests were less sensitive than a qualitative RNA assay and only detected HIV Ag when the viral load was high. The BioPlex assay detected acute infection in about half of the cases, and identified most of those infections as Ag positive/Ab negative.

AB - Background Assays that detect HIV antigen (Ag) and antibody (Ab) can be used to screen for HIV infection. Objectives To compare the performance of the BioPlex 2200 HIV Ag-Ab assay and two other Ag/Ab combination assays for detection of acute HIV infection. Study design Samples were obtained from 24 individuals (18 from the US, 6 from South Africa); these individuals were classified as having acute infection based on the following criteria: positive qualitative RNA assay; two negative rapid tests; negative discriminatory test. The samples were tested with the BioPlex assay, the ARCHITECT HIV Ag/Ab Combo test, the Bio-Rad GS HIV Combo Ag-Ab EIA test, and a viral load assay. Results Twelve (50.0%) of 24 samples had RNA detected only (> 40 to 13,476 copies/mL). Ten (43.5%) samples had reactive results with all three Ag/Ab assays, one sample was reactive with the ARCHITECT and Bio-Rad assays, and one sample was reactive with the Bio-Rad and BioPlex assays. The 11 samples that were reactive with the BioPlex assay had viral loads from 83,010 to >750,000 copies/mL; 9/11 samples were classified as Ag positive/Ab negative by the BioPlex assay. Conclusions Detection of acute HIV infection was similar for the BioPlex assay and two other Ag/Ab assays. All three tests were less sensitive than a qualitative RNA assay and only detected HIV Ag when the viral load was high. The BioPlex assay detected acute infection in about half of the cases, and identified most of those infections as Ag positive/Ab negative.

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