Performance of swabs, lavage, and diluents to quantify biomarkers of female genital tract soluble mucosal mediators

Charlene S. Dezzutti, Craig Hendrix, Jeanne M. Marrazzo, Zhenyu Pan, Lei Wang, Nicolette Louissaint, Sabah Kalyoussef, N. Merna Torres, Florian Hladik, Urvi Parikh, John Mellors, Sharon L. Hillier, Betsy C. Herold

Research output: Contribution to journalArticle

Abstract

Background: Measurement of immune mediators and antimicrobial activity in female genital tract secretions may provide biomarkers predictive of risk for HIV-1 acquisition and surrogate markers of microbicide safety. However, optimal methods for sample collection do not exist. This study compared collection methods. Methods: Secretions were collected from 48 women (24 with bacterial vaginosis [BV]) using vaginal and endocervical Dacron and flocked swabs. Cervicovaginal lavage (CVL) was collected with 10 mL of Normosol-R (n = 20), saline (n = 14), or water (n = 14). The concentration of gluconate in Normosol-R CVL was determined to estimate the dilution factor. Cytokine and antimicrobial mediators were measured by Luminex or ELISA and corrected for protein content. Endogenous anti-HIV-1 and anti-E. coli activity were measured by TZM-bl assay or E. coli growth. Results: Higher concentrations of protein were recovered by CVL, despite a 10-fold dilution of secretions, as compared to swab eluents. After protein correction, endocervical swabs recovered the highest mediator levels regardless of BV status. Endocervical and vaginal flocked swabs recovered significantly higher levels of anti-HIV-1 and anti-E. coli activity than Dacron swabs (P

Original languageEnglish (US)
Article numbere23136
JournalPLoS One
Volume6
Issue number8
DOIs
StatePublished - 2011

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Therapeutic Irrigation
female genitalia
Biomarkers
Human immunodeficiency virus 1
Escherichia coli
Bacterial Vaginosis
HIV-1
biomarkers
Polyethylene Terephthalates
anti-infective agents
secretion
Dilution
Proteins
Anti-Infective Agents
Assays
cytokines
proteins
Enzyme-Linked Immunosorbent Assay
methodology
protein content

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Performance of swabs, lavage, and diluents to quantify biomarkers of female genital tract soluble mucosal mediators. / Dezzutti, Charlene S.; Hendrix, Craig; Marrazzo, Jeanne M.; Pan, Zhenyu; Wang, Lei; Louissaint, Nicolette; Kalyoussef, Sabah; Torres, N. Merna; Hladik, Florian; Parikh, Urvi; Mellors, John; Hillier, Sharon L.; Herold, Betsy C.

In: PLoS One, Vol. 6, No. 8, e23136, 2011.

Research output: Contribution to journalArticle

Dezzutti, CS, Hendrix, C, Marrazzo, JM, Pan, Z, Wang, L, Louissaint, N, Kalyoussef, S, Torres, NM, Hladik, F, Parikh, U, Mellors, J, Hillier, SL & Herold, BC 2011, 'Performance of swabs, lavage, and diluents to quantify biomarkers of female genital tract soluble mucosal mediators', PLoS One, vol. 6, no. 8, e23136. https://doi.org/10.1371/journal.pone.0023136
Dezzutti, Charlene S. ; Hendrix, Craig ; Marrazzo, Jeanne M. ; Pan, Zhenyu ; Wang, Lei ; Louissaint, Nicolette ; Kalyoussef, Sabah ; Torres, N. Merna ; Hladik, Florian ; Parikh, Urvi ; Mellors, John ; Hillier, Sharon L. ; Herold, Betsy C. / Performance of swabs, lavage, and diluents to quantify biomarkers of female genital tract soluble mucosal mediators. In: PLoS One. 2011 ; Vol. 6, No. 8.
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