TY - JOUR
T1 - Performance of focus ELISA tests for HSV-1 and HSV-2 antibodies among university students with no history of genital herpes
AU - Mark, Hayley D.
AU - Nanda, Joy P.
AU - Roberts, Jessica
AU - Rompalo, Anne
AU - Melendez, Johan H.
AU - Zenilman, Jonathan
PY - 2007/9
Y1 - 2007/9
N2 - OBJECTIVES: To define the performance characteristics of the Focus ELISA HSV-1 and HSV-2 assay among 100 university students. STUDY DESIGN: HSV-1 and HSV-2 Focus ELISA and Western Blot assays were performed on sera from university students who reported no history of genital herpes. RESULTS: HSV-2 and HSV-1 seroprevalence by Western Blot were 3.4% and 48%, respectively. In this population, the positive predictive value of the Focus HSV-2 ELISA was 37.5%, the sensitivity was 100%, and specificity was 94.1%. The PPV of the Focus HSV-1 ELISA was 96.7%, the sensitivity was 69.0%, and the specificity was 97.8%. CONCLUSIONS: In this low-prevalence population, the positive predictive value of the Focus HSV-2 ELISA test was low. This finding, together with those reported elsewhere, indicates that caution is warranted when recommending HSV screening in low-prevalence or heterogeneous populations. Consideration should be given to raising the cutoff index value for defining a positive test result.
AB - OBJECTIVES: To define the performance characteristics of the Focus ELISA HSV-1 and HSV-2 assay among 100 university students. STUDY DESIGN: HSV-1 and HSV-2 Focus ELISA and Western Blot assays were performed on sera from university students who reported no history of genital herpes. RESULTS: HSV-2 and HSV-1 seroprevalence by Western Blot were 3.4% and 48%, respectively. In this population, the positive predictive value of the Focus HSV-2 ELISA was 37.5%, the sensitivity was 100%, and specificity was 94.1%. The PPV of the Focus HSV-1 ELISA was 96.7%, the sensitivity was 69.0%, and the specificity was 97.8%. CONCLUSIONS: In this low-prevalence population, the positive predictive value of the Focus HSV-2 ELISA test was low. This finding, together with those reported elsewhere, indicates that caution is warranted when recommending HSV screening in low-prevalence or heterogeneous populations. Consideration should be given to raising the cutoff index value for defining a positive test result.
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U2 - 10.1097/01.olq.0000258307.18831.f0
DO - 10.1097/01.olq.0000258307.18831.f0
M3 - Article
C2 - 17457239
AN - SCOPUS:34548190328
SN - 0148-5717
VL - 34
SP - 681
EP - 685
JO - Sexually transmitted diseases
JF - Sexually transmitted diseases
IS - 9
ER -