Performance of Five Commercial Identification Platforms for Identification of Staphylococcus delphini

Matthew C. Canver, Tsigereda Tekle, Samantha T. Compton, Katrina Callan, Eileen M. Burd, Barbara L. Zimmer, David A. Bemis, Karen C. Carroll, Lars F. Westblade

Research output: Contribution to journalArticle

Abstract

The Staphylococcus intermedius group (SIG) is a collection of coagulase-positive staphylococci consisting of four distinct species, namely, Staphylococcus cornubiensis, Staphylococcus delphini, Staphylococcus intermedius, and Staphylococcus pseudintermedius SIG members are animal pathogens and rare causes of human infection. Accurate identification of S. pseudintermedius has important implications for interpretation of antimicrobial susceptibility testing data and may be important for other members of the group. Therefore, we sought to evaluate the performance of five commercially available identification platforms with 21 S. delphini isolates obtained from a variety of animal and geographic sources. Here, we show that automated biochemical platforms were unable to identify S. delphini to the species level, a function of its omission from their databases, but could identify isolates to the SIG level with various degrees of success. However, all automated systems misidentified at least one isolate as Staphylococcus aureus One matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system was able to identify S. delphini to the species level, suggesting that MALDI-TOF MS is the best option for distinguishing members of the SIG. With the exception of S. pseudintermedius, it is unclear if other SIG members should be routinely identified to the species level; however, as our understanding of their role in animal and human diseases increases, it may be necessary and important to do so.

Original languageEnglish (US)
JournalJournal of clinical microbiology
Volume57
Issue number11
DOIs
StatePublished - Nov 1 2019

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Common Dolphins
Staphylococcus intermedius
Staphylococcus
Mass Spectrometry
Lasers
Animal Diseases
Coagulase
Staphylococcus aureus
Databases
Infection

Keywords

  • automated biochemical platform
  • coagulase-positive staphylococci
  • MALDI-TOF MS
  • Staphylococcus delphini
  • Staphylococcus intermedius group

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Canver, M. C., Tekle, T., Compton, S. T., Callan, K., Burd, E. M., Zimmer, B. L., ... Westblade, L. F. (2019). Performance of Five Commercial Identification Platforms for Identification of Staphylococcus delphini. Journal of clinical microbiology, 57(11). https://doi.org/10.1128/JCM.00721-19

Performance of Five Commercial Identification Platforms for Identification of Staphylococcus delphini. / Canver, Matthew C.; Tekle, Tsigereda; Compton, Samantha T.; Callan, Katrina; Burd, Eileen M.; Zimmer, Barbara L.; Bemis, David A.; Carroll, Karen C.; Westblade, Lars F.

In: Journal of clinical microbiology, Vol. 57, No. 11, 01.11.2019.

Research output: Contribution to journalArticle

Canver, MC, Tekle, T, Compton, ST, Callan, K, Burd, EM, Zimmer, BL, Bemis, DA, Carroll, KC & Westblade, LF 2019, 'Performance of Five Commercial Identification Platforms for Identification of Staphylococcus delphini', Journal of clinical microbiology, vol. 57, no. 11. https://doi.org/10.1128/JCM.00721-19
Canver, Matthew C. ; Tekle, Tsigereda ; Compton, Samantha T. ; Callan, Katrina ; Burd, Eileen M. ; Zimmer, Barbara L. ; Bemis, David A. ; Carroll, Karen C. ; Westblade, Lars F. / Performance of Five Commercial Identification Platforms for Identification of Staphylococcus delphini. In: Journal of clinical microbiology. 2019 ; Vol. 57, No. 11.
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